脊髓切片中运动神经元的膜片箝技术研究:一种分析药物作用的高效工具(英文)
Patch clamp studies of motor neurons in spinal cord slices: a tool for high-resolution analysis of drug actions作者机构：CA 94305 Department of Anesthesia Stanford University School of Medicine USA
出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))
年 卷 期：2000年第21卷第6期
页 面：29-37页
核心收录：
学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学]
基 金：National Institute of Neurological Disorders and Stroke NINDS: R01NS013108
摘 要：AIM: To develop a tool for detailed analysis of spirally acting anesthetic and analgesic agents. METHODS: Studies were done on visually identified motor neurons in 400 uuuuuuuum thick spinal cord slices from 14-23 d old rats using patch clamp techniques. Ethanol was used as a prototype general anesthetic agent. RESULTS: Cell bodies in the ventrolateral horn identified as motor neurons by retrograde fluorescent labeling had a mean dimension of 32 ± 5 /Mm (x ± s, n = 25). Mean resting potential was - 62. 8 ± 2. 4 mV; input resistance was 44±24 MΩ (n = 19). Threshold was -44± 7 mV, and action potential amplitude 101 ± 9 mV from baseline. Ethanol concentrations at and below 50-200 mmol/L decreased motor neuron excitability to the injected current; there was no effect on resting potential, but a variable reversible increase in input resistance. Ethanol re-versibly depressed the excitatory postsynaptic potential, with a dose-response relationship similar to that previously observed for the population e
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