Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence

作     者：CHEN LIU MICHAEL MALEK IVAN POON LANZHOU JIANG ARIF MSIDDIQUEE COLIN JRSHEPPARD ANN ROBERTS HARRY QUINEY DOUGUO ZHANG XIAOCONG YUAN JIAO LIN CHRISTIAN DEPEURSINGE PIERRE MARQUET SHAN SHAN KOU 

作者机构：Nanophotonics Research CentreShenzhen Key Laboratory of Micro-Scale Optical Information TechnologyShenzhen UniversityShenzhen518060China Department of Chemistry and PhysicsLa Trobe Institute for Molecular Science(LIMS)La Trobe UniversityMelbourneVictoria3086Australia Department of Optics and Optical EngineeringAnhui Key Laboratory of Optoelectronic Science and TechnologyUniversity of Science andTechnology of ChinaHefei230026China Department of Biochemistry and GeneticsLa Trobe Institute for Molecular Science(LIMS)La Trobe UniversityMelbourneVictoria3086Australia Australian Research Council(ARC)Centre of Excellence in Advanced Molecular ImagingAustralia Istituto Italiano di TecnologiaGenova16163Italy School of PhysicsUniversity of MelbourneVictoria3010Australia School of EngineeringRMIT UniversityMelbourneVictoria3001Australia Microvision and Microdiagnostic Group(SCI STI CHD)Ecole Polytechnique Federale de Lausanne(EPFL)1015LausanneSwitzerland Laboratory for Cellular Imaging and EnergeticsBiological and Environmental Sciences and Engineering DivisionKing Abdullah University ofScience and Technology(KAUST)Thuwal23955-6900Saudi Arabia Joint International Research Unit in Neurodevelopment and Child PsychiatryCHUVDepartement de PsychiatrieLausanneSwitzerlandUniversiteLavalQuebecCanada Institut universitaire en santementale de QuebecQuebecCanada Centre d’optiquephotonique et laserDepartment of Psychiatry and NeuroscienceUniversiteLaval2375rue de la TerrasseQuebecQC G1V0A6Canada 

出 版 物：《Photonics Research》 (光子学研究（英文版）)

年 卷 期：2019年第7卷第9期

页      面：1042-1050页

核心收录：

学科分类：070207[理学-光学] 07[理学] 08[工学] 070104[理学-应用数学] 0803[工学-光学工程] 0701[理学-数学] 0702[理学-物理学] 

基　　金：Australian Research Council(ARC)(DE120102352) National Natural Science Foundation of China(NSFC)(61427819) Shenzhen Science and Technology Innovation Commission(KQCS2015032416183980) Government of Guangdong Province(00201505) Schweizerischer Nationalfonds zur Forderung der Wissenschaftlichen Forschung(SNF)(149652) 

主　　题：Fourier red Carlo Monte image Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence 

摘      要：We report a dual-contrast method of simultaneously measuring and visualizing the volumetric structural information in live biological samples in three-dimensional(3D) space. By introducing a direct way of deriving the 3D scattering potential of the object from the synthesized angular spectra, we obtain the quantitative subcellular morphology in refractive indices(RIs) side-by-side with its fluorescence signals. The additional contrast in RI complements the fluorescent signal, providing additional information of the targeted zones. The simultaneous dual-contrast 3D mechanism unveiled interesting information inaccessible with previous methods, as we demonstrated in the human immune cell(T cell) experiment. Further validation has been demonstrated using a Monte Carlo model.