Hypothermic intervention for 3 hours inhibits apoptosis in neonatal rats with hypoxic-ischemic brain damage

作     者：Yale Guo Zhankui Li Ruilin Li Baoshan Su Shaoping Huang Jianping Zhou 

作者机构：Department of Pediatrics Second Affiliated Hospital of Medical College of Xi＇an Jiaotong University Xi＇an 710004 Shaanxi Province China Department of Neonatology Maternal and Child Health Hospital of Shaanxi Province Xi＇an 710004 Shaanxi Province China Department of Pathology Second Affifiated Hospital of Medical College of Xi＇an Jiaotong University Xi＇an 710004 Shaanxi Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2011年第6卷第22期

页      面：1709-1714页

核心收录：

学科分类：0710[理学-生物学] 090603[农学-临床兽医学] 071010[理学-生物化学与分子生物学] 1002[医学-临床医学] 081704[工学-应用化学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0906[农学-兽医学] 

主　　题：hypothermia hypoxia ischemia neural protection neural regeneration 

摘      要：A neonatal rat model of hypoxic-ischemic brain damage was designed and implemented in this study. Rats were subjected to hypothermia at 31℃ immediately following hypoxia-ischemia for either 3, 6 or 15 hours. TdT-mediated dUTP nick end labeling demonstrated that the number of apoptotic cells was reduced in the rat cerebral cortex, hippocampus and periventricular white matter following hypothermia. Immunohistochemistry revealed that Bcl-2 and p16 expression were decreased. Inhibition of apoptosis was greatest with the 3 hour hypothermic treatment, followed by hypothermia for 6 hours. In contrast, hypothermia for 15 hours led to a decrease in neuronal number in the cerebral cortex. The results demonstrate that hypothermic intervention at 31℃ protects brain tissue against hypoxic-ischemic brain damage by inhibiting apoptosis, and that the optimal length of treatment is 3 hours.