葡萄糖氧化酶催化荧光测定的非酶体系研究

作     者：李庆阁 许金钩 黄贤智 陈国珍 

作者机构：厦门大学肿瘤细胞工程国家专业实验室厦门大学化学系厦门361005 

出 版 物：《高等学校化学学报》 (Chemical Journal of Chinese Universities)

年 卷 期：1997年第18卷第1期

页      面：52-54页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

主　　题：葡萄糖氧化酶 荧光测定 羟基自由基 非酶体系 

摘      要：A novel non-enzymatic method for the determination of oxidases and their sub-strates was proposed. The principle was examined by fluorometric determination of glucose oxidase(GOD). Using EGTA (NH4)2Fe (SO4), as modified Fenton reagent, H2O2, the prod-uct of GOD catalytic reaction was converted into hydroxyl radical, the later oxidized tereph-thalic acid, which was chosen from four fluorogenic indicator of hydroxyl radical, into fluo-rescent product GOD was thus measured according to the reaction rates. The rnethod is sim-ple, stable, cheap and at least as sensitive as its counterpart enzymatic method-