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高等学校化学学报 1997年第1期18卷 52-54页

作者：李庆阁许金钩黄贤智陈国珍厦门大学肿瘤细胞工程国家专业实验室厦门大学化学系厦门361005

A novel non-enzymatic method for the determination of oxidases and their sub-strates was proposed. The principle was examined by fluorometric determination of glucose oxidase(GOD). Using EGTA (NH4)2Fe (SO4), as modified Fenton reagent, H2O2, the prod-uct of GOD catalytic reaction was converted into hydroxyl radical, the later oxidized tereph-thalic acid, which was chosen from four fluorogenic indicator of hydroxyl radical, into fluo-rescent product GOD was thus measured according to the reaction rates. The rnethod is sim-ple, stable, cheap and at least as sensitive as its counterpart enzymatic method-

关键词：葡萄糖氧化酶荧光测定羟基自由基非酶体系

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