Construction of the glucose isomerase deficient strain of Streptomyces M1033 by homologous recombination

作     者：XU Chong LIAO Jun XIE Wen CHENG Yang ZHU Guoping YANG Yonghui CHEN Chenglu TAO Limei NIU Liwen WANG Yuzhen 

作者机构：Department of Cell and Molecular BiologySchool of Life SciencesUniversity of Science and Technology of ChinaHefei 230026China Key Laboratory of Structural BiologyUniversity of Science and Technology of ChinaHefei 230026China 

出 版 物：《Chinese Science Bulletin》 (Chin. Sci. Bull.)

年 卷 期：2000年第45卷第1期

页      面：64-70页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

基　　金：国家科技攻关项目 

主　　题：glucose isomerase Streptomyces homologous recombination gene disruption. 

摘      要：After the establishment of the transformation conditions of Streptomyces diastaticus No.7 Strain M1033, the integration plasmid pXW for homologous recombination, which contains a 600 bp fragment of incomplete Gl (G138P. G247D) gene, has been constructed in order to realize the stable overexpression of the Gl (G138P. G247D) which is valuable for large-scale industrial production. The G/gene’s disruption has been realized by pXW’s integration into M1033 chromosomes via homologous recombination and Gl deficient strain of Streptomyces M1033 has been obtained. The reliability of introduction of mutation has been proved by analysis of recombinant fragment and affirmance of existence of the mutation, as well as detection of the stability of the deficient strain.