Inf luence of Spirulina platensis exudates on the endocrine and nervous systems of a mammalian model

作     者：Samah Mamdouh Mohamed Fathy Ashraf Mohamed Mohamed Essa 

作者机构：Zoology Department Faculty of Science Fayoum University Biology Department Faculty of Science Jazan University Botany Department Faculty of Science Fayoum University 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2015年第5卷第6期

页      面：449-455页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1006[医学-中西医结合] 10[医学] 100602[医学-中西医结合临床] 

主　　题：Spirulina platensis Sterol-like compounds Sex hormones Antioxidant enzymes Neurotransmitters 

摘      要：Objective: To investigate the ef ect of intra-peritoneal injection of purii ed exudates of axenic Spirulina platensis on the mammalian endocrine and nervous systems. Methods: The intra-peritoneal injection of the cyanobacterial exudates in mice was applied. Sex hormonal levels of testosterone and progesterone were measured using radioimmunoassay while the follicular stimulating hormone and luteinizing hormone were evaluated by direct chemiluminescence. In addition, superoxide dismutase, catalase and glutathione peroxidase were monitored in the hippocampus region using spectrophotometric method. The levels of the hippocampal monoamines, dopamine, noradrenaline and serotonin were analyzed by high performance liquid chromatography while the acetyl choline neurotransmitter was measured by colorimetric method using choline/acetylcholine assay kit. Results: A sharp disruption in the sex hormones levels of testosterone, progesterone, follicular stimulating hormone and luteinizing hormone was demonstrated in the serum of the treated mice. At the same time, a signii cant reduction in the endogenous antioxidant defense enzymes, superoxide dismutase, catalase and glutathione peroxidase was observed in the hippocampus region of the injected mice. Moreover, levels of dopamine, noradrenaline, serotonin and acetyl choline neurotransmitter in the same region were signii cantly af ected as a result of the treatment with Spirulina i ltrate. The gas chromatography-mass spectrometer and liquid chromatography mass spectrometry/mass spectrometry analysis showed the presence of some sterol-like compounds in the cyanobacterial i ltrate. Conclusions: This study demonstrated the capability of Spirulina to release detrimental bioactive metabolites into their surrounding that can disrupt the mammalian endocrine and nervous systems.