cis-acting element located in the bovine foamy virus internal promoter possesses the properties of a transcrip-tional enhancer

作     者：Wentao Qiao Chunguang Guo Shuhui Wang Jinzhong Wang Qimin Chen Yunqi Geng 

作者机构：Nankai Univ Coll Life Sci Tianjin 300071 Peoples R China 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2002年第47卷第13期

页      面：1108-1112页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学] 

基　　金：This work was supported by the National Natural Science Foundation of China (Grant No. 39970033 ) Doctoral Fund in High School (Grant No. 98005516) 

主　　题：bovine foamy virus (BFV) internal promoter (IP) Tas responsive element (TRE) enhancer. 

摘      要：Bovine foamy virus encodes a transcriptional transactivitor, Tas or Borf-1, which governs the level of viral transcripts initiated by both the promoter in the long terminal repeat (LTR) and the internal promoter (IP) located in the env gene through their cis-acting targets. We have identified and characterized a 72 bp TBS (Borf-1) responsive element located in BFV3026, internal promoter (TREIP) by deletion mutant and transient expression assay. This cis-acting target element in the internal promoter has the properties of a transcriptional enhancer which functions independently of its orientation, position and also in heterologous promoters (BFV LTR and bovine immunodeficiency virus, BIV LTR). Alignments reveal that there are positional similarity and sequence homology among BFV TREIP, SFV-1 TREIP proximal element and SFV-3 TREIPH, which suggests that this kind of cis-acting elements possesses some common functional character.