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Virologica Sinica 2020年第1期35卷 73-82页

作者： Shanshan Xu Liujun Chen Yinglian Tang Peipei Yuan Jun Yan Yingcheng Zheng Li Huang Zhi Li Yan Sun Song Han Jun Yin Qin Pan Biwen Peng Xiaohua He Wanhong Liu Department of Allergy Zhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Immunology and Hubei Province Key Laboratory of Allergy and Immunology School of Basic Medical SciencesWuhan UniversityWuhan 430071China Hubei Provincial Key Laboratory of Developmentally Originated Disease Wuhan 430071China College of Life Sciences Shanxi Normal UniversityXi’an 710062China Centre for Medical Research Wuhan UniversityWuhan 430071China

Prototype foamy virus(PFV)is a unique retrovirus that infects animals and humans and does not cause clinical *** noncoding RNAs(lncRNAs)are believed to exert multiple regulatory functions during viral ***,we utilized RNA sequencing(RNA-seq)to characterize and identify the lncRNA lnc-RP5-1086 D14.3.1-1:1(lnc-RP5),which is markedly decreased in PFV-infected ***,little is known about the function of lnc-RP5 during PFV *** this study,we identified lnc-RP5 as a regulator of the PFV transcriptional transactivator(Tas).Lnc-RP5 enhanced the activity of the PFV internal promoter(IP).The expression of PFV Tas was found to be promoted by ***,mi R-129-5 p was found to be involved in the lnc-RP5-mediated promotion of PFV IP activity,while the Notch1 protein suppressed the activity of PFV IP and the expression of *** results demonstrate that lnc-RP5 promotes the expression of PFV Tas through the miR-129-5 p/Notch1/PFV IP *** work provides evidence that host lnc RNAs can manipulate PFV replication by employing mi RNAs and proteins during an early viral infection.

关键词： Prototype foamy virus(PFV) lnc-RP5 Transactivator(Tas) Internal promoter miR-129-5p

Borf-1 protein identified as a transcriptional trans-activator of bovine foamy virus

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Chinese Science Bulletin 1999年第11期44卷 1017-1021页

作者： Jiajian Liu Shuhong Liu Qimin Chen Yunqi Geng Nankai Univ Coll Life Sci Tianjin 300071 Peoples R China

Bovine foamy virus (BFV), a member of the spumavirus subfamily of retroviruses,contains two open reading frames (ORF-1 and ORF-2) in addition to the genes coding for gag,po/and env. Borf-1 protein, encoded by BFV ORF-1, is identified as a transcriptional transactivator, which augments gene expression directed by the viral long terminal repeat (LTR).Further investigations in transient expression assays reveal that the Borf-1 responsive elements are located in the U3 domain of the LTR, upstream from position -140 ( + 1 represents the transcription initiation site), and the BFV RU5 region has an inhibitory effect in LTR-directed gene expression.

关键词： bovine foamy virus (BFV )3026 China Strain transcriptional trans-activator transient expression assay.

cis-acting element located in the bovine foamy virus internal promoter possesses the properties of a transcrip-tional enhancer

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Chinese Science Bulletin 2002年第13期47卷 1108-1112页

作者： Wentao Qiao Chunguang Guo Shuhui Wang Jinzhong Wang Qimin Chen Yunqi Geng Nankai Univ Coll Life Sci Tianjin 300071 Peoples R China

Bovine foamy virus encodes a transcriptional transactivitor, Tas or Borf-1, which governs the level of viral transcripts initiated by both the promoter in the long terminal repeat (LTR) and the internal promoter (IP) located in the env gene through their cis-acting targets. We have identified and characterized a 72 bp TBS (Borf-1) responsive element located in BFV3026, internal promoter (TREIP) by deletion mutant and transient expression assay. This cis-acting target element in the internal promoter has the properties of a transcriptional enhancer which functions independently of its orientation, position and also in heterologous promoters (BFV LTR and bovine immunodeficiency virus, BIV LTR). Alignments reveal that there are positional similarity and sequence homology among BFV TREIP, SFV-1 TREIP proximal element and SFV-3 TREIPH, which suggests that this kind of cis-acting elements possesses some common functional character.

关键词： bovine foamy virus (BFV) internal promoter (IP) Tas responsive element (TRE) enhancer.

Making and Study of Moderate Pressure Glow Discharge on the Basis of Electrolytic foamy Cathode, Prepared as the Aqueous Solution of NaHCO3

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Journal of Modern Physics 2010年第4期1卷 236-243页

作者： Dmitry V. Vyalykh Alexander E. Dubinov Igor L. L’vov Sergey A. Sadovoy Victor D. Selemir 不详

A method to generate the dynamic moderate pressure dc glow discharge on the basis of electrolytic cathode in the form of aqueous solution of sodium bicarbonate NaHCO3 is described. Photo and video images of the discharge are presented as well as the synchronized therewith “oscillograms” of current and voltage. Different phases of the discharge were discovered, one of which is a quasi-stationary glow discharge with the foamy cathode, was recorded for the first time. It was shown, that in this phase the discharge is supported by a so-called three-dimensional cathode spot, having the finite volume. The time-spatial diagram was plotted for the discharge. The Rayleigh-Taylor instability in the two-layered electrolytic cathode was recorded.

关键词： Glow Discharge Electrolytic Cathode Aqueous Solution Sodium Bicarbonate foamy Cathode Rayleigh-Taylor Instability

The pathology of unusual subtypes of prostate cancer

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Chinese Journal of Cancer Research 2016年第1期28卷 130-143页

作者： Jing Li Zhe Wang State Key Laboratory of Cancer Biology Department of Pathology Xi Jing Hospital Fourth Military Medical University

There are some current literatures describing the morphologic change of prostate carcinoma variants. Some subtypes do not respond to hormone deprivation therapy, for example adenosquamous and squamous cell carcinoma （SQCC）, basaloid and adenoid cystic carcinoma （ACC）, small cell carcinoma （SmCC）, sarcomatoid carcinoma, urothelial carcinoma; some are defined in special Gleason grade, some develop different prognosis. So, it is very important to identify these rare subtypes to avoid misdiagnosis. In this review, we aim to describe the typical clinicopathological features of the rare variants of prostate cancer, including prostate acinar adenocarcinoma morphologic variants.

关键词： Prostate carcinoma subtype ductal neuroendocrine (NE) squamous mucinous foamy pseudo hyperplastic