Anthelmintic activity of Securidaca longepedunculata(Family:Polygalaceae)root extract in mice,in vitro and in vivo

作     者：Adiele RC Fakae BB Isuzu IU 

作者机构：Department of Anatomy and Cell Biology.Division of Biomedical SciencesCollege of MedicineUniversity of SaskatchewanSaskatoon.SK.S7N5E Cameco MS Neuroscience Research CenterSaskatoon City HospitalSaskatoonSKS7K OM7 Department of Veterinary Parasitology and EntomologyFaculty of Veterinary MedicineUniversity of NigeriaNsukka Vice Chancellor's OfficeRiver State university of Science and TechnologyPort Harcourt Department of Veterinary Physiology and PharmacologyFaculty of Veterinary MedicineUniversity of NigeriaNsukka Deputy Vice Chancellor's Office.University of NigeriaNsukka 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2013年第6卷第11期

页      面：841-846页

核心收录：

学科分类：090603[农学-临床兽医学] 1002[医学-临床医学] 09[农学] 0906[农学-兽医学] 

主　　题：Anthelmintic Securidaca longepedunculata Haemonchus contortus Heligmosomoides polygyrus Brine shrimps Larvicidal Fecal egg count 

摘      要：Objective:To elucidate the pharmacological bases of oral administration of Securidaca longepedunculata(***) root extract as an anthelmintic in folkloric medicine. Methods:Albino mice were infected with infective third(L3) larval stage of Heligmosomoides polygyrus(***) by esophageal *** establishment of the adult worms in the intestine,the mice were treated with 0-2 000 mg/kg body weight(bw) of methanolic root extract of *** and 100 mg/kg bw of pyrantel embonate,the reference drug in vivo. Bioactivity and larvicidal effects of the extract were tested by exposing brine shrimps(Artemia salina) to 0.00-1.00 mg/mL and the L3 stage of Heligmosomoides contortus(***) and *** to 0.00-2.50 mg/mL of the extract in ***:The percentage yield of the extract was 7.13%w/w dry *** brine shrimps toxicity bioassay resulted in an LC_(50) of 74.18 μ g/*** extract had a significant,dose-dependent larvicidal effect on the L3 stage of *** and *** with the terminal effect of 75%and 70%at the highest exposure concentrations,*** extract however,did not affect the number of worm eggs per gram(epg) of fecal materials(P0.05) and total worm burden(twb) of adult *** in infected *** with pyrantel embonate significant reduced both the fecal egg count and twb to 0 compared to the untreated control(P0.05).Conclusions:These results indicate that *** root extract contains potent bioactive compounds and has larvicidal effect on L3 stage of *** and ***,substantiating its use as anthelmintic in alternative medicine.