Highly sensitive and selective electrochemical immunosensors by substrate-enhanced electroless deposition of metal nanoparticles onto three-dimensional graphene@Ni foams

作     者：Changrong Zhao Xiaoli Li Shixia An Dongliang Zheng Shuaili Pei Xiao Zheng Yu Liu Qingqing Yao Mei Yang Liming Dai 

作者机构：Institute of Advanced Materials for Nano-Bio Applications School of Ophthalmology ■ Optometry Wenzhou Medical University Wenzhou 325027 China Department of Macromolecule Science and Engineering Case Western Reserve University Cleveland OH 44106 USA 

出 版 物：《Science Bulletin》 (科学通报（英文版）)

年 卷 期：2019年第64卷第17期

页      面：1272-1279页

核心收录：

学科分类：07[理学] 08[工学] 

基　　金：financially supported by the Natural Science Foundation of Zhejiang Province (LY18H200008) Wenzhou Science and Technology Bureau Project (Y20170203) National Natural Science Foundation of China (51433005) 

主　　题：Electrochemical immunosensor Substrate-enhanced electroless deposition Graphene Ni foam Metal nanoparticles a-Fetoprotein antigen 

摘      要：In this study, we have for the first time preformed the facile substrate-enhanced electroless deposition(SEED) of metal nanoparticles onto monolithic graphene@Ni foams for construction of disposable three-dimensional(3 D) electrochemical immunosensors. Specifically, we firstly used the SEED method to deposit gold nanoparticles(AuNPs) onto the graphene@Ni foam for immobilization of antibody(Ab1). This is followed by a second step SEED deposition to produce silver nanoparticles(AgNPs) for electrochemical stripping detection. Using a-fetoprotein antigen(AFP) as a module analyte, the newly-developed sensor showed a wide linear response, ranging from 5.0 pg/mL to 5.0 ng/mL and a low detection limit down to 2.3 pg/mL. The newly-developed 3 D-immunosensor is sensitive, reliable,and easy to be fabricated, showing great potential for clinic applications.