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Electrochemical Immunosensor for a-fetoprotein Based on Gold Nanoparticles/Graphene-Prussian Blue

Chinese Journal of Chemistry 2012年第3期30卷 485-490页

作者：王广凤张戈黄昊王伦 Anhui Normal Univ Coll Chem&Mat SciKey Lab Funct Mol SolidsKey Lab Chem BiosensingWuhu 241000AnhuiPeoples R China Hefei Univ Technol Key Lab Controllable Chem React&Mat Chem EngnHefei 230009AnhuiPeoples R China

The electrochemical immunosensor for a-fetoprotein （AFP） was fabricated based on the platform of gold nanoparticles （GNP）/graphene （Gr）-prussian blue （PB）. By electrodeposition, GNP were modified on the surface of the prepared Gr-PB. The anti-AFP-1, l＇-ferrocenedicarboxylic acid （FcDA） as label was directly immobilized on the platform of GNP/Gr-PB. And after the immunoreactions, the formed complex inhibited the electron transfer and decreased the catalytic current of FcDA toward the reduction of H2O2. And in the range of 10-3200 pgomL^-1, the decreased current is linear with the concentration of AFP, with a detection limit of 3 ***-1. The developed im- munoassay method showed good precision, high sensitivity, acceptable stability and reproducibility, and could be used for the detection of real samples with consistent results in comparison with those obtained by the enzyme linked immunosorbent assay （ELISA） method.

关键词： a-fetoprotein electrochemical immunosensor graphene ferrocene prussian blue antigens immuno-assay

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α-fetoprotein involvement during glucocorticoid-induced precocious maturation in rat colon

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World Journal of Gastroenterology 2011年第24期17卷 2933-2940页

作者： Min Chen Peng Sun Xiao-Yan Liu Dan Dong Jun Du Luo Gu Ying-Bin Ge Lian Yun Gang Higher Vocational Technical College of Traditional Chinese Medicine Lianyungang 222006 Jiangsu Province China Department of Physiology Nanjing Medical University Nanjing 210029 Jiangsu Province China The Laboratory Center for Basic Medical Sciences Nanjing Medical University Nanjing 210029 Jiangsu Province China

AIM： TO investigate the role of a-fetoprotein （AFP）, a cancer-associated fetal glycoprotein, in glucocorticoidinduced precocious maturation in rat colon. METHODS： Colons from suckling Sprague-Dawley rats were used in this study. Corticosterone acetate at a dose of 100 μg/g body weight was given to normal pups on days 7, 9 and 11 after birth to induce hypercorticoidism. Control animals were injected with identical volumes of normal saline. Some rats receiving corticosterone 7 d after birth were also treated with mifepristone （RU38486）, a glucocorticoid cytoplasm receptor antagonist to investigate the effects of glucocorticoids （GCs）. The morphological changes of the crypt depth and villous height of the villous zone in colon were observed as indicesof colon maturation. Expression levels of AFP in colons were detected by reverse transcriptase polymerase chain reaction and Western blotting. To identify the cellular lo- calization of AFP in developing rat colons, double-immu- nofluorescent staining was performed using antibodies to specific mesenchymal cell marker and AFP. RESULTS： Corticosterone increased the crypt depth and villous height in the colon of 8- and 10-d-old rats with hypercorticoidism compared with that in the control ani- mals （120% in 8-d-old rats and 118% in 10-d-old rats in villous height, P = 0.021; 145% in 8-d-old rats and 124% in 10-d-old rats in crypt depth, P = 0.017）. These increases were accompanied by an increase of AFP ex- pression in both mRNA and protein （2.5-folds in 8-d- old and 2.5-folds in 10-d-old rats higher than in control animals, P = 0.035; 1.8-folds in 8-d-old and 1.3-folds in 10-d-old rats higher than in control animals, P = 0.023）. Increased crypt depth and villous height and increased expression of AFP in the colon of rats with hypercorti- coidism were blocked by mifepristone. Both had positive staining for AFP or vimentin, and overlapped in mesen- chymal cells at each tested colon. CONCLUSION： GCs promote the development of ra

关键词： Glucocorticoids a-fetoprotein Precociousmaturation Colon Rat

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Comparison of chemiluminescence enzyme immunoassay based on magnetic microparticles with traditional colorimetric ELISA for the detection of serum α-fetoprotein

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Journal of Pharmaceutical Analysis 2012年第2期2卷 130-135页

作者： Qian-Yun Zhang a,b,Hui Chen a,Zhen Lin a,Jin-Ming Lin a a Beijing Key Laboratory of Microanalytical Methods and Instrumentation,Department of Chemistry,Tsinghua University,Beijing 100029,China b Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China Beijing Key Laboratory of Microanalytical Methods and Instrumentation Department of Chemistry Tsinghua University Beijing 100029 China Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China

A chemiluminescence enzyme immunoassay based on magnetic microparticles (MmPs-CLEIA) was developed to evaluate serum a-fetoprotein (AFP) in parallel with traditional colorimetric enzyme-linked immunosorbent assay (ELISA).A systematic comparison between the MmPs-CLEIA and colorimetric ELISA concluded that the MPs-CLEIA exhibited fewer dosages of immunoreagents,less total assay time,and better linearity,recovery,precision,sensitivity and *** was detected in forty human serum samples by the proposed MPs-CLEIA and ELISA,and the results were compared with commercial electrochemiluminescence immunoassay (ECLIA) *** correlation coefficient between MPs-CLEIA and ELISA was obtained with R 2 0.6703;however,the correlation between MPs-CLEIA and ECLIA (R 2 0.9582) was obviously better than that between colorimetric ELISA and ECLIA (R 2 0.6866).

关键词： a-fetoprotein Hepatocellular carcinoma Chemiluminescence enzyme immunoassay Magnetic microparticles Colorimetric enzyme-linked immunosorbent assay

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Highly sensitive and selective electrochemical immunosensors by substrate-enhanced electroless deposition of metal nanoparticles onto three-dimensional graphene@Ni foams

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Science Bulletin 2019年第17期64卷 1272-1279页

作者： Changrong Zhao Xiaoli Li Shixia An Dongliang Zheng Shuaili Pei Xiao Zheng Yu Liu Qingqing Yao Mei Yang Liming Dai Institute of Advanced Materials for Nano-Bio Applications School of Ophthalmology ■ Optometry Wenzhou Medical University Wenzhou 325027 China Department of Macromolecule Science and Engineering Case Western Reserve University Cleveland OH 44106 USA

In this study, we have for the first time preformed the facile substrate-enhanced electroless deposition(SEED) of metal nanoparticles onto monolithic graphene@Ni foams for construction of disposable three-dimensional(3 D) electrochemical immunosensors. Specifically, we firstly used the SEED method to deposit gold nanoparticles(AuNPs) onto the graphene@Ni foam for immobilization of antibody(Ab1). This is followed by a second step SEED deposition to produce silver nanoparticles(AgNPs) for electrochemical stripping detection. Using a-fetoprotein antigen(AFP) as a module analyte, the newly-developed sensor showed a wide linear response, ranging from 5.0 pg/mL to 5.0 ng/mL and a low detection limit down to 2.3 pg/mL. The newly-developed 3 D-immunosensor is sensitive, reliable,and easy to be fabricated, showing great potential for clinic applications.

关键词： Electrochemical immunosensor Substrate-enhanced electroless deposition Graphene Ni foam Metal nanoparticles a-fetoprotein antigen

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Case Report of an Endodermal Sinus Vaginal Tumor in an Infant Girl

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Chinese Journal of Clinical Oncology 2007年第1期4卷 71-72页

作者： Chunyu Sun YUqiu Cui Hongfei Li Fengying Wang Department of Pathology Fushun No. 3 Hospital Fushun 113004 China

Case Report An female infant patient, aged 8 months old, suffered from irregular colporrhagia for a period of 1 month after which she was taken to our hospital on 30th April, 2003. A pelvic CT examination displayed a 6.5 cm×3.0 cm shadow of a soft-tissue tumor growing longitudinally in her supravaginal uterine area （Fig. 1）. The density of the shadow was uneven, in which there were irregular low-density loci, an indication of a compression of the colon and bladder and a diffuse boundary between the posterior wall of the urinary bladder and tumor. No abnormalities were found in either kidney or ovary, the liver or gall bladder. Also no obvious lesions were seen on the chest X-ray film, and routine blood and urine laboratory examinations were normal.

关键词： endodermal sinus tumor a-fetoprotein diagnosis.

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