Protective effects of lipoic acid-niacin dimers against blue light-induced oxidative damage to retinal pigment epithelium cells

作     者：Xiu-Lan Zou Yong-Zhen Yu Hong-Hua Yu Guan-Feng Wang Rong-Biao Pi Zhe Xu Chu Zhang Wen-Jie Zhou Dan-Dan Li Xuan-Ge Chen Yu-Ping Zou 

作者机构：Department of OphthalmologyGeneral Hospital of SouthernTheatre Command of PLAGuangzhou510010GuangdongProvinceChina Department of OphthalmologyGuangdong ProvincialPeople’s HospitalGuangzhou510000Guangdong ProvinceChina Department of OphthalmologyThird Affiliated Hospitalof Guangzhou Medical UniversityGuangzhou510000Guangdong ProvinceChina School of Pharmaceutical SciencesSun Yat-sen UniversityGuangzhou510000Guangdong ProvinceChina Department of Ophthalmologythe First Affiliated HospitalGuangdong Pharmaceutical UniversityGuangzhou510000Guangdong ProvinceChina Zhongshan Ophthalmic Centre of Sun Yat-sen UniversityGuangzhou510000Guangdong ProvinceChina 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2019年第12卷第8期

页      面：1262-1271页

核心收录：

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：Supported by the Guangzhou Science and Technology Foundation of Guangdong Province (No.2014J4100035 No.2014KP000071) 

主　　题：lipoic acid-niacin dimers retinal pigment epithelium cell lipoic acid oxidative stress reactive oxygen species apoptosis 

摘      要：AIM: To evaluate the protective effects of lipoic acid-niacin(N2 L) dimers against blue light(BL)-induced oxidative damage to human retinal pigment epithelium(hRPE) cells in ***: h RPE cells were divided into a control group(CG), a BL group, an N2 L plus BL irradiation group, an α-lipoic acid(ALA) plus BL group, an ALA-only group, and an N2 L-only group. hRPE cellular viability was detected by performing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium(MTT) bromide assays, and apoptosis was evaluated by annexin-V-PE/7-AAD staining followed by flow cytometry. Ultrastructural changes in subcellular organelles were observed by transmission electron microscopy. Reactive oxygen species formation was assayed by flow cytometry. The expression levels of the apoptosis-related proteins BCL-2 associated X protein(BAX), B-cell leukmia/lymphoma 2(BCL-2), and caspase-3 were quantified by Western blot ***: BL exposure with a light density of 4±0.5 mW/cm2 exceeding 6 h caused hRPE toxicity, whereas treatment with a high dose of N2 L(100 mol/L) or ALA(150 mol/L) maintained cell viability at control levels. BL exposure caused vacuole-like degeneration, mitochondrial swelling, and reduced microvillus formation;however, a high dose of N2 L or ALA maintained the ultrastructure of hRPE cells and their organelles. High doses of N2 L and ALA also protected hRPE cells from BL-induced apoptosis, which was confirmed by Western blot analysis: BCL-2 expression significantly increased, while BAX and caspase-3 expression slightly decreased compared to the ***: High-dose N2 L treatment(100 mol/L) can reduce oxidative damage in degenerating hRPE cells exposed to BL with an efficacy similar to ALA.