Lymphocyte subsets in alcoholic liver disease

作     者：Luís Costa Matos Paulo Batista Nuno Monteiro Joo Ribeiro Maria A Cipriano Pedro Henriques Fernando Giro Armando Carvalho 

作者机构：Faculty of Medicine of the University of Coimbra3004-504 CoimbraPortugal Centro Hospitalar TondelaViseu E.P.E3504-509 ViseuPortugal Department of AnatomopathologyUniversity of Coimbra Hospital3004-504 CoimbraPortugal 

出 版 物：《World Journal of Hepatology》 (世界肝病学杂志（英文版）（电子版）)

年 卷 期：2013年第5卷第2期

页      面：46-55页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by Centro Hospitalar Tondela Viseu E.P.E. Portugal (public hospital) 

主　　题：Lymphocyte subsets Flow cytometry Alcoholism Alcoholic liver disease Liver fibrosis Liver biopsy HFE gene 

摘      要：AIM:To compare lymphocyte subsets between healthy controls and alcoholics with liver ***:The patient cohort for this study included individuals who were suspected to have alcoholic liver disease(ALD) and who had undergone liver biopsy(for disease grading and staging,doubts about diagnosis,or concurrent liver disease;n = 56).Normal controls included patients who were admitted for elective cholecystectomy due to non-complicated gallstones(n = 27).Formalin-fixed,paraffin-embedded liver biopsy specimens were sectioned and stained with hematoxylin and eosin and Perls Prussian *** non-alcoholic steatohepatitis score was used to assess markers of *** population subsets were determined by flow cytometry.T lymphocytes were identified(CD3+),and then further subdivided into CD4+ or CD8+ populations.B lymphocytes(CD19+) and natural killer(NK) cell numbers were also *** addition to assessing lymphocyte subpopulation differences between ALD patients and controls,we also compared subsets of alcoholic patients without cirrhosis or abstinent cirrhotic patients to normal ***:The patient cohort primarily consisted of older *** alcoholism was present in 66.1%.Reported average daily alcohol intake was 164.9 g and the average lifetime cumulative intake was 2211.6 *** was present in 39.3% of the patients and 66.1% had significant fibrosis(perisinusoidal and portal/periportal fibrosis,bridging fibrosis,or cirrhosis) in their liver *** average Mayo end-stage liver disease score was *** hereditary hemochromatosis genotypes were *** patients(n = 56) presented with significant lymphopenia(1.5 × 109/L ± 0.5 × 109/L vs 2.1 × 109/L ± 0.5 × 109/L,P 0.0001),due to a decrease in all lymphocyte subpopulations,except for NK lymphocytes:CD3+(1013.0 ± 406.2/mm3 vs 1523.0 ± 364.6/mm3,P 0.0001),CD4+(713.5 ± 284.7/mm3 vs 992.4 ± 274.7/mm3,P 0.0001),CD8+(262.3 ± 140.4/mm3 vs 478.9 ± 164.6/mm3,P 0.0001),and CD19+(120.6