Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progression

作     者：Jan Nevoral Lukas Landsmann Miriam Stiavnicka Petr Hosek Jiri Moravec Sarka Prokesova Hedvika Rimnacova Eliska Koutna Pavel Klein Kristyna Hoskova Tereza Zalmanova Tereza Fenclova Jaroslav Petr Milena Kralickova 

作者机构：Biomedical CenterFaculty of Medicine in PilsenCharles Universityalej Svobody 1655/7632300 PilsenCzech Republic Department of Histology and EmbryologyFaculty of Medicine in PilsenCharles UniversityKarlovarska 4830166 PilsenCzech Republic Faculty of ScienceCharles UniversityAlbertov 2038/612800 PragueCzech Republic Faculty of AgricultureFood and Natural ResourcesCzech University of Life Sciences in PragueKamycka 12916500 Praha-SuchdolCzech Republic Institute of Animal SciencePratelstvi 815/10710400Prague 10-UhrinevesCzech Republic 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2019年第10卷第4期

页      面：895-906页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 07[理学] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 071007[理学-遗传学] 0703[理学-化学] 0836[工学-生物工程] 090501[农学-动物遗传育种与繁殖] 

基　　金：supported by the Charles University Research Fund(Progres Q39) the National Sustainability Programme I(NPU I)Nr.LO1503 provided by the Ministry of Education Youth and Sports of the Czech Republic(MEYS CR) project No.SVV 02690 awarded by MEYS CR the project No.CZ.02.1.01/0.0/0.0/16_019/0000787 “Fighting Infectious Diseases” awarded by MEYS CR and financed from The European Regional Development Fund supported by the National Agency of Agriculture Sciences(NAZV QJ1510138) the Czech Ministry of Agriculture(MZe RO 0718) 

主　　题：Epigenetics Histone code In vitro maturation Oocyte SIRT1 Sirtuin 1 

摘      要：Background: SIRT1 histone deacetylase acts on many epigenetic and non-epigenetic targets. It is thought that SIRT1 is involved in oocyte maturation;therefore, the importance of the ooplasmic SIRT1 pool for the further fate of mature oocytes has been strongly suggested. We hypothesised that SIRT1 plays the role of a signalling molecule in mature oocytes through selected epigenetic and non-epigenetic ***: We observed SIRT1 re-localisation in mature oocytes and its association with spindle *** mature oocytes, SIRT1 distribution shows a spindle-like pattern, and spindle-specific SIRT1 action decreasesα-tubulin acetylation. Based on the observation of the histone code in immature and mature oocytes, we suggest that SIRT1 is mostly predestined for an epigenetic mode of action in the germinal vesicles(GVs) of immature oocytes. Accordingly, BML-278-driven trimethylation of lysine K9 in histone H3 in mature oocytes is considered to be a result of GV epigenetic ***: Taken together, our observations point out the dual spatiotemporal SIRT1 action in oocytes,which can be readily switched from the epigenetic to non-epigenetic mode of action depending on the progress of meiosis.