Cytotoxic effect of methanolic extracts of Fritillaria imperialis bulbs and Eryngium caucasicum leaves on hepatoma and colon cancer cells

作     者：Mostafa Kardan Zahra Yazdani Zaher Morsaljahan Mohammad Ali Ebrahimzadeh Alireza Rafiei 

作者机构：Student Research CommitteeDepartment of ImmunologyMolecular and cell Biology Research CenterSchool of MedicineMazandaran University of Medical SciencesSariIran Department of ImmunologySchool of MedicineMazandaran University of Medical SciencesSariIran Pharmaceutical Sciences Research CenterDepartment of Medicinal ChemistryFaculty of PharmacyMazandaran University of Medical SciencesSariIran 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2019年第9卷第8期

页      面：353-358页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by deputy of Research and Technology Mazandaran University of Medical Sciences 

主　　题：Fritillaria imperialis Eryngium caucasicum Cytotoxity Colon cancer Hepatoma cancer 

摘      要：Objective: To evaluate antitumor activities of Fritillaria imperialis and Eryngium caucasicum methanolic extracts on human hepatoma (HepG2) and colon cancer (HCT116) cell lines in comparison to human foreskin fibroblasts as the normal cells. Methods: Methanolic extracts of Fritillaria imperialis and Eryngium caucasicum were prepared by the maceration method. The effect of the extracts at various concentrations (100, 200, 400, 600, and 800 μg/mL) on cell survival was evaluated using the MTT method. Besides, fluorescence staining was used to evaluate death patterns of the cells. Results: MTT assay showed that Fritillaria imperialis significantly decreased the viability of all cell lines after 24 and 48 hours of treatments. However, Eryngium caucasicum extract did not show any significant cytotoxicity effect on the cell lines. Fluorescence staining revealed that Fritillaria imperialis induced apoptosis of HCT116 cells at 550 μg/mL. Conclusions: Fritillaria imperialis extract has antiproliferative and cytotoxic effects on HCT116 and HepG2 cancer cells and therefore, may serve as an anticancer agent.