Analysis of Amino Acids in Houttuynia Cordata Thunb. by High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD)

作     者：Xuan FENG Zhannan YANG Zhu ZHENG Juan HU Xiaofang WEI Honghao HUO Cheng ZHAO Quling ZHANG 

作者机构：Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment of Guizhou ProvinceGuizhou Normal University School of Life SciencesGuizhou Normal University 

出 版 物：《Medicinal Plant》 (药用植物：英文版)

年 卷 期：2019年第10卷第2期

页      面：28-34页

学科分类：10[医学] 

基　　金：Supported by National Natural Science Foundation of China(41761010,81260641) Science and Technology Foundation of Guizhou Province of China(1121) Projects of Guizhou Normal University for Scientific Research of Doctors 

主　　题：Houttuynia cordata Thunb. Amino acid High performance liquid chromatography Medicinal plant 

摘      要：[Objectives] The biologically active compounds of Houttuynia cordata Thunb. as a medicinal and edible plant are well documented. However, amino acid as an important indicator of the value of H. cordata is little reported. [Methods] Amino acids of the H. cordata were analysed by pre-column derivatization with 2,4-dinitrochlorobenzne(CDNB) coupled to HPLC/DAD. The optimized conditions of the amino acids derivatized by CDNB were followed: 110 ℃ of hydrolysis temperature, 1∶12 of the equivalent proportion between amino acid and CDNB, 80 min of derivatization reaction time and 80 ℃ of derivatization reaction temperature. The method was used to determine the amino acids of H. cordata samples from 11 different geographical sites in Guizhou. [Results] The results suggested that the contents of 17 amino acids were quite variable at different H. cordata samples. [Conclusions] This study will provide a basis for the quality evaluation of H. cordata nutrition.