检索结果-南通市图书馆

Directional screening and identification of potential cytotoxic components from Achnatherum inebrians by a combination of surface palsmon resonance and chromatography

在线全文

学校读者我要写书评

暂无评论

Chinese Herbal Medicines 2023年第2期15卷 329-336页

作者： Wenbin Zhou Man Wang Aiqin Zhang Danrong Huang Hua Guo Gangyi Shen Key Laboratory of Ethnomedicine(Minzu University of China) Ministry of EducationSchool of PharmacyMinzu University of ChinaBeijing 100081China College of Life and Environmental Sciences Minzu University of ChinaBeijing 100081China

Objective:To establish a method for directional screening of the cytotoxic components from the medicinal herb of Achnatherum inebrians by a combination of surface plasmon resonance(SPR)biosensor and chromatographic isolation technology.Methods:Under the guidance of bioactive assessment based on binding abilities between objects and the a-Mannosidase(a-Man)target,the active components from different solvents extracts,different polar extraction parts and fractions were screened orderly and directionally using SPR.Components with a high binding ability to a-Man can be precisely oriented in a narrower fractions range and are easy to isolate.Three human cancer cells were used to evaluate the cytotoxic activity of component with the highest affinity to a-Man.Results:Eight compounds were isolated and identificated from A.inebrians for the first time.Deoxyvasicinone possessed the highest affinity to a-Man among them.Moreover,deoxyvasicinone showed good effects on inhibited proliferation of human hepatoma cells HepG2(IC_(50)=5.7 μmol/L),human breast cancer cells MCF7(IC_(50)=7.21 μmol/L)and human lung cancer cells HCC827(IC_(50)=0.75 μmol/L),respectively.In particular,its inhibitory effect on HCC827 was stronger than the positive drug gefitinib(IC_(50)=1.65 μmol/L).Conclusion:A comprehensive strategy of directional screening potential cytotoxic components from herb based on biomolecular interaction and chromatography was established.Deoxyvasicinone as an effective anti-cancer component was initially isolated from A.inebrians.It is expected that this screening strategy could provide new perspectives for rapid screening and identification of active components from natural plants with the complex matrix.

关键词： Achnatherum inebrians(Hance)Keng chromatography cytotoxic components deoxyvasicinone surface plasmon resonance α-Mannosidase

Chip-based ion chromatography (chip-IC) with a sensitive five-electrode conductivity detector for the simultaneous detection of multiple ions in drinking water

在线全文

学校读者我要写书评

暂无评论

Microsystems & Nanoengineering 2020年第1期6卷 530-537页

作者： Xiaoping Li Honglong Chang Ministry of Education Key Laboratory of Micro/Nano Systems for Aerospace School of Mechanical EngineeringNorthwestern Polytechnical University710072 Xi’anP.R.China

The emerging need for accurate,efficient,inexpensive,and multiparameter monitoring of water quality has led to interest in the miniaturization of benchtop chromatography systems.This paper reports a chip-based ion chromatography(chip-IC)system in which the microvalves,sample channel,packed column,and conductivity detector are all integrated on a polymethylmethacrylate(PMMA)chip.A laser-based bonding technique was developed to guarantee simultaneous robust sealing between the homogeneous and heterogeneous interfaces.A five-electrode-based conductivity detector was presented to improve the sensitivity for nonsuppressed anion detection.Common anions(F^(−),Cl^(−),NO_(3)^(−),and SO _(4)^(2−))were separated in less than 8 min,and a detection limit(LOD)of 0.6 mg L^(−1) was achieved for SO_(4)^(2−).Tap water was also analyzed using the proposed chip-IC system,and the relative deviations of the quantified concentration were less than 10%when compared with that a commercial IC system.

关键词： conductivity electrode chromatography

A liquid chromatography with tandem mass spectrometry method for quantitating total and unbound ceritinib in patient plasma and brain tumor

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2018年第1期8卷 20-26页

作者： Xun Bao Jianmei Wu Nader Sanai Jing Li Karmanos Cancer Institute Wayne State University Barrow Neurological Institute St. Joseph's Hospital& Medical Center

A rapid, sensitive, and robust reversed-phase liquid chromatography with tandem mass spectrometry method was developed and validated for the determination of total and unbound ceritinib, a secondgeneration ALK inhibitor, in patient plasma and brain tumor tissue samples. Sample preparation involved simple protein precipitation with acetonitrile. Chromatographic separation was achieved on a Waters ACQUITY UPLC BEH C_(18) column using a 4-min gradient elution consisting of mobile phase A(0.1% formic acid in water) and mobile phase B(0.1% formic acid in acetonitrile), at a flow rate of 0.4 m L/min. Ceritinib and the internal standard([^(13)C_6]ceritinib) were monitored using multiple reaction monitoring mode under positive electrospray ionization. The lower limit of quantitation(LLOQ) was 1 n M of ceritinib in plasma. The calibration curve was linear over ceritinib concentration range of 1–2000 n M in plasma. The intra-and interday precision and accuracy were within the generally accepted criteria for bioanalytical method( o15%).The method was successfully applied to assess ceritinib brain tumor penetration, as assessed by the unbound drug brain concentration to unbound drug plasma concentration ratio, in patients with brain tumors.

关键词： Ceritinib Reversed-phase liquid chromatography with tandem mass spectrometry (LC–MS/MS) Fraction unbound in plasma Fraction unbound in brain tissue Brain tumor penetration Unbound brain-to-plasma partition coefficient

Identification of the active ingredients from Guangtongxiao decoction in rat bile based on ultra-performance liquid chromatography/Synapt G2 quadrupole time-of-flight tandem mass spectrometry

在线全文

学校读者我要写书评

暂无评论

Journal of Traditional Chinese Medicine 2020年第6期40卷 999-1006页

作者： Deng Zhihao Yan Yan Zhao Baoming Wang Rui Wang Xiaofeng Chen Haoxuan Wen Binyu Dongzhimen Hospital Beijing University of Chinese MedicineBeijing 100700China Dongfang Hospital Beijing University of Chinese MedicineBeijing 100078China

OBJECTIVE:To identify the active ingredients and metabolites in rat bile after Guangtongxiao decoction(GTX)had been administered via the rectal route.METHODS:Drug-containing bile samples were collected via a catheter in the bile duct and could be used 5 h after rectal administration.The main active components and their metabolites in rat bile following rectal administration of GTX were identified and analyzed using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry.RESULTS:Positive and negative modes were applied to analyze and identify the chemical ingredients in the bioactive fractions of GTX.Eight peaks were identified by comparison with the standard compounds:berberine hydrochloride,dehydrocorydaline,tetrahydropalmatine,corydaline,magnoflorine,magnolol,obacunone and albiflorin.Furthermore,60 metabolites were detected in rat bile based on mass-fragmentation behaviors,and 21 metabolites were reported for the first time.CONCLUSION:Our findings provide a solid basis for further pharmacologic and pharmacokinetic studies of GTX.

关键词： chromatography high performance liquid Flight mass spectrometry Bile Metabolites Guangtongxiao decoction

Offline two-dimensional liquid chromatography coupled with ion mobility-quadrupole time-of-flight mass spectrometry enabling fourdimensional separation and characterization of the multicomponents from white ginseng and red ginseng

在线全文

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2020年第6期10卷 597-609页

作者： Tiantian Zuo Chunxia Zhang Weiwei Li Hongda Wang Ying Hu Wenzhi Yang Li Jia Xiaoyan Wang Xiumei Gao Dean Guo Tianjin State Key Laboratory of Modern Chinese Medicine Tianjin University of Traditional Chinese Medicine312 Anshanxi RoadTianjin300193China Tianjin Key Laboratory of TCM Chemistry and Analysis Tianjin University of Traditional Chinese Medicine312 Anshanxi RoadTianjin300193China Shanghai Research Center for Modernization of Traditional Chinese Medicine National Engineering Laboratory for TCM Standardization TechnologyShanghai Institute of Materia MedicaChinese Academy of Sciences501 Haike RoadShanghai201203China

Inherent complexity of plant metabolites necessitates the use of multi-dimensional information to accomplish comprehensive profiling and confirmative identification.A dimension-enhanced strategy,by offline two-dimensional liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry(2 D-LC/IM-QTOF-MS)enabling four-dimensional separations(2 D-LC,IM,and MS),is proposed.In combination with in-house database-driven automated peak annotation,this strategy was utilized to characterize ginsenosides simultaneously from white ginseng(WG)and red ginseng(RG).An offline 2 DLC system configuring an Xbridge Amide column and an HSS T3 column showed orthogonality 0.76 in the resolution of ginsenosides.Ginsenoside analysis was performed by data-independent high-definition MSE(HDMSE)in the negative ESI mode on a Vion?IMS-QTOF hybrid high-resolution mass spectrometer,which could better resolve ginsenosides than MSEand directly give the CCS information.An in-house ginsenoside database recording 504 known ginsenosides and 58 reference compounds,was established to assist the identification of ginsenosides.Streamlined workflows,by applying UNIFI?to automatedly annotate the HDMSEdata,were proposed.We could separate and characterize 323 ginsenosides(including 286 from WG and 306 from RG),and 125 thereof may have not been isolated from the Panax genus.The established 2 D-LC/IM-QTOF-HDMSEapproach could also act as a magnifier to probe differentiated components between WG and RG.Compared with conventional approaches,this dimensionenhanced strategy could better resolve coeluting herbal components and more efficiently,more reliably identify the multicomponents,which,we believe,offers more possibilities for the systematic exposure and confirmative identification of plant metabolites.

关键词： Dimension-enhanced strategy Multicomponent characterization Ginsenoside Offline two-dimensional liquid chromatography Ion mobility-quadrupole time-of-flight mass spectrometry In-house database

Advances of supercritical fluid chromatography in lipid profiling

在线全文

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2019年第1期9卷 1-8页

作者： Yang Yang Yanshan Liang Jina Yang Fengying Ye Ting Zhou Gongke Li School of Biology and Biological Engineering South China University of Technology School of Chemistry Sun Yat-sen University

Supercritical fluid chromatography(SFC) meets with great favor due to its high efficiency, low organic solvent consumption, and the specialty for the identification of the isomeric species. This review describes the advances of SFC in targeted and untargeted lipid profiling. The advancement of the SFC instruments and the stationary phases are summarized. Typical applications of SFC to the targeted and untargeted lipid profiling are discussed in detail. Moreover, the perspectives of SFC in the lipid profiling are also proposed. As a useful and promising tool for investigating lipids in vitro and in vivo, SFC will predictably obtain further development.

关键词： Supercritical fluid chromatography Online SFC technique Untargeted lipid profiling Targeted lipid profiling Lipidomics

Study on screening potential allergenic proteins from infant milk powders based on human mast cell membrane chromatography and histamine release assays

在线全文

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2019年第1期9卷 55-61页

作者： Ping Zhang Yingdi Shi Xiaoshuang He Wei Sun Yanni Lv Xiaofang Hou School of Pharmacy Xi'an Jiaotong University Linyi Institute for Food and Drug Control Shaanxi Engineering Research Center of Cardiovascular Drugs Screening & Analysis

Cow's milk allergy is mainly observed in infants and young children. Most allergic reactions affect the skin, followed by the gastrointestinal and respiratory systems. Conventional diagnosis is based on positive allergy studies and evaluation of parameters including IgE and IgG1 levels, acute allergic skin response and anaphylactic shock reactions. We developed a cell membrane chromatographic(CMC)method based on human mast cells(HMC-1) for screening potential allergens in infant formula milk powders(IFMP). HMC-1 cell membranes were extracted and mixed with silica to prepare cell membrane chromatography columns(10 mm ? 2 mm i.d., 5 mm). Under the conditions of 0.2 mL/min flow rate and214 nm detection wavelength, human breast milk showed no retention. However, IFMP showed clear retention. The retained fractions were collected and analyzed through matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS). Four major milk proteins, i.e., α-casein, β-casein, α-lactalbumin, and β-lactoglobulin A, were identified. Furthermore, these proteins and β-lactoglobulin B showed clear retention on HMC-1/CMC columns. To test the degranulation effects of the five proteins, histamine and β-hexosaminidase release assays were carried out. All five proteins induced HMC-1 cells to release histamine and β-hexosaminidase. Also, we established a reversed phase liquid chromatographic(RPLC) method for the determination of the five proteins in IFMP and the results showed that 90% proteins in IFMP were α-casein and β-casein. We concluded that cow's milk proteins may be potential allergens and caseins cause more β-casein allergic risk than other proteins. This conclusion was consistent with other studies.

关键词： Allergenic proteins Cell membrane chromatography Milk powders

Simultaneous determination of amino acids in different teas using supercritical fluid chromatography coupled with single quadrupole mass spectrometry

在线全文

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2019年第4期9卷 254-258页

作者： Yang Huang Tiejie Wang Marianne Fillet Jacques Crommen Zhengjin Jiang Institute of Pharmaceutical Analysis College of Pharmacy Jinan University Guangzhou 510632 China Shenzhen Institute for Drug Control Shenzhen China Department of Pharmaceutics State Key Laboratory of Natural Medicines China Pharmaceutical University Nanjing China Laboratory for the Analysis of Medicine Department of Pharmaceutical Sciences CIRM University of Liege CHU B36 B-4000 Liege Belgium

Tea is a widely consumed beverage and has many important physiological properties and potential health benefits. In this study, a novel method based on supercritical fluid chromatography coupled with mass spectrometry (SFC-MS) was developed to simultaneously determine 11 amino acids in different types of tea (green teas, Oolong tea, black tea and Pu-erh tea). The separation conditions for the analysis of the selected amino acids including the column type, temperature and backpressure as well as the type of additive, were carefully optimized. The best separation of the 11 amino acids was obtained by adding water (5%, v/v) and trifluoroacetic acid (0.4%, v/v) to the organic modifier (methanol). Finally, the developed SFC-MS method was fully validated and successfully applied to the determination of these amino acids in six different tea samples. Good linearity (r ≥ 0.993), precision (RSDs≤ 2.99%), accuracy (91.95%-107.09%) as well as good sample stability were observed. The limits of detection ranged from 1.42 to 14.69 ng/mL, while the limits of quantification were between 4.53 and 47.0 ng/mL. The results indicate that the contents of the 11 amino acids in the six different tea samples are greatly influenced by the degree of fermentation. The proposed SFC-MS method shows a great potential for further investigation of tea varieties.

关键词： Amino acids Teas Supercritical fluid chromatography with single quadrupole mass spectrometry (SFCMS)

Quantitative analysis by reversed-phase high-performance liquid chromatography and retinal neuroprotection after topical administration of moxonidine

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2020年第3期13卷 390-398页

作者： Qian Zhang Mei-Fang Chu Yan-Hong Li Chun-Hua Li Run-Jia Lei Si-Cen Wang Bao-Jun Xiao Jian-Gang Yang Department of Ophthalmology Xi’an Fourth HospitalShaanxi Ophthalmological HospitalXi’an 710004Shaanxi ProvinceChina Xi’an Eye Hospital First Affiliated Hospital of Northwest UniversityXi’an No.1 HospitalXi’an 710002Shaanxi ProvinceChina School of Pharmacy Xi’an Jiaotong UniversityXi’an 710068Shaanxi ProvinceChina West Street Community Health Center of Lianhu District Xi’an 710002Shaanxi ProvinceChina

AIM:To determine moxonidine in aqueous humor and iris-ciliary body by reversed-phase high performance liquid chromatography(RP-HPLC),and to evaluate the retinal neuroprotective effect after topical administration with moxonidine in a high intraocular pressure(IOP)model.METHODS:The eyes of albino rabbits were administered topically and ipsilaterally with 0.2%moxonidine.A RPHPLC method was employed for the identification and quantification of moxonidine between 2 and 480 min,which presented in the aqueous humor and iris-ciliary body.Flash electroretinography(F-ERG)amplitude and superoxide dismutase(SOD)level were measured between day 1 and day 15 after topical administration with moxonidine in a rabbit model of high IOP.Histological and ultrastructural observation underwent to analyze the changes of retinal morphology,the inner retinal layers(IRL)thickness,and retinal ganglion cell(RGC)counting.RESULTS:Moxonidine was detectable between 2 and 480 min after administration,and the peak concentration developed both in the two tissues at 30 min,0.51μg/m Lin aqueous humor and 1.03μg/g in iris-ciliary body.In comparison to control,F-ERG b-wave amplitude in moxonidine eyes were significantly differences between day 3 and day 15(P<0.01)in the high IOP model;SOD levels were significantly higher at all time-points(P<0.01)with a maximum level of 20.29 U/mgprot at day 15;and RGCs were significantly higher(P<0.05).CONCLUSION:Moxonidine is a viable neuroprotective agent with application to high IOP model.All layers of retina,including RGC layer,retinal nerve fiber layer and INL,are more preserved after moxonidine administration.SOD plays a neuroprotective role in ocular hypertension-mediated RGC death.

关键词： reversed-phase high-performance liquid chromatography moxonidine retinal ganglion cell neuroprotection superoxide dismutase