Identification of the active ingredients from Guangtongxiao decoction in rat bile based on ultra-performance liquid chromatography/Synapt G2 quadrupole time-of-flight tandem mass spectrometry

作     者：Deng Zhihao Yan Yan Zhao Baoming Wang Rui Wang Xiaofeng Chen Haoxuan Wen Binyu Deng Zhihao;Yan Yan;Zhao Baoming;Wang Rui;Wang Xiaofeng;Chen Haoxuan;Wen Binyu

作者机构：Dongzhimen HospitalBeijing University of Chinese MedicineBeijing 100700China Dongfang HospitalBeijing University of Chinese MedicineBeijing 100078China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2020年第40卷第6期

页      面：999-1006页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 10[医学] 100602[医学-中西医结合临床] 

基　　金：Supported by the Youth Program of National Natural Science Foundation of China(To Investigate the Spasmolysis of Intestinal Absorption Solution of Guang Tong Xiao Aqueous Extract&Effective Components for Anal Sphincter Based on Calcium Channels No.81704077)。 

主　　题：Chromatography high performance liquid Flight mass spectrometry Bile Metabolites Guangtongxiao decoction 

摘      要：OBJECTIVE:To identify the active ingredients and metabolites in rat bile after Guangtongxiao decoction(GTX)had been administered via the rectal route.METHODS:Drug-containing bile samples were collected via a catheter in the bile duct and could be used 5 h after rectal administration.The main active components and their metabolites in rat bile following rectal administration of GTX were identified and analyzed using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry.RESULTS:Positive and negative modes were applied to analyze and identify the chemical ingredients in the bioactive fractions of GTX.Eight peaks were identified by comparison with the standard compounds:berberine hydrochloride,dehydrocorydaline,tetrahydropalmatine,corydaline,magnoflorine,magnolol,obacunone and albiflorin.Furthermore,60 metabolites were detected in rat bile based on mass-fragmentation behaviors,and 21 metabolites were reported for the first time.CONCLUSION:Our findings provide a solid basis for further pharmacologic and pharmacokinetic studies of GTX.