Preliminary studies for a new antibiotic from the marine mollusk Melo melo(Lightfoot,1786)

作     者：Kanagasabapathy Sivasubramanian Samuthirapandian Ravichandran Kumaresan M 

作者机构：Centre of Advanced Study in Marine BiologyAnnamalai University 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2011年第4卷第4期

页      面：310-314页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1002[医学-临床医学] 10[医学] 

主　　题：Antibacterial Antifungal TLC SDS-PAGE Melo melo 

摘      要：Objective:To search for new bioactive compounds from marine mollusks Melo melo(***). Methods:Preliminary work for bioactive compound was identified by using disc diffusion methods against human *** analyses of compound were done by using TLC, *** also estimate the amount of protein in the samples by following Biuret ***:In antibacterial activity the maximum diameter of 24 mm zone of inhibition was recorded against Klebsiella pneumoniae(***) strain of the mucus extract and minimum zone of inhibition of 11 mm was observed in Salmonella typhi(***) strain of body tissue *** antifungal activity of the extraction shows maximum activity against Trichophyton mentagarophytes(***)(14 mm) and minimum activity was recorded in Aspergillus flavus(***)(11 mm).The extract of mucus,nerve tissue,body tissue and kidney that showed antimicrobial activity was subjected to TLC to determine the presence of the peptides and amide groups,and also subjected to SDS-PACE to estimate the molecular weight of proteins in a clear band were detected in the gel that represented kidney,body tissue,brain and mucus represent 14,17,22,45 ***:The extracts from marine mollusks *** is the potential source of producing bioactive compounds against human pathogens and can be used for synthesis of new drugs.