Bioactivity of compounds isolated from the leaves of the Lantana camara Linn plant

作     者：Suryati Elida Mardiah Mai Efdi Kartika MZ Yuni Mala Sari Suryati;Elida Mardiah;Mai Efdi;Kartika MZ;Yuni Mala Sari

作者机构：Department of Chemistry Faculty of Mathematics and Sciences Andalas University 

出 版 物：《Journal of Chinese Pharmaceutical Sciences》 (中国药学（英文版）)

年 卷 期：2019年第28卷第5期

页      面：360-368页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Universitas Andalas leaders of Andalas University 

主　　题：Lantana camara Linn Lantadene B Glycosides flavonoid MCF-7 breast cell cancer Cytotoxic Antioxidant activity 

摘      要：Two compounds with different bioactivities were isolated from the leaves of the Lantana camara Linn *** 1 was triterpenoid(Lantadene B)(22β-dimethylacroyloxy-3-oxoolean-12-en-28-oic-acid,C_(35)H_(52)O_5),which was isolated from n-hexane fraction and obtained as white solid(amorphous).Compound 2 was glycoside flavonoid(5-hydroxy-6,4′-dimethoxy flavon-7-O-glucopyranose,C_(23)H_(24)O_(11)),which was isolated from ethyl acetate and obtained as pale yellow solid(amorphous).Extraction was performed with maceration method using methanol ***,fractionation was carried out using n-hexane and ethyl acetate *** and purification of both fractions were conducted using chromatography *** structure of the isolated compounds was determined through spectral analyses of ultraviolet(UV),infrared(IR),and nuclear magnetic resonance(~1H NMR and ^(13)C NMR),distortionless enhancement by polarization transfer(DEPT),heteronuclear multiple bond connectivity(HMBC),heteronuclear single quantum correlation(HSQC),and H-H COSY(~1H-~1H homonuclear correlated spectroscopy).The cytotoxic activity of compound 1 was tested against MCF-7 breast cancer cells in vitro using MTT assay(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide),showing very strong cytotoxic activity in inhibiting the growth of MCF-7 breast cancer cells,and the IC_(50) value was 1.1336μ*** antioxidant activity of compound 2 was tested using DPPHassay(1,1-diphenyl-2-picrylhydrazyl),showing strong activity in inhibiting free radicals,and its IC_(50) value was 71.03 mg/L.