BASIC FIBROBLAST GROWTH FACTOR GENE TRANSFECTION TO ENHANCE THE REPAIR OF AVASCULAR NECROSIS OFTHE FEMORAL HEAD

作     者：CaoYang Shu-huaYang Jing-yuanDu JinLi Wei-huaXu Yu-fangXiong 

作者机构：DepartmentofOrthopedicsUnionHospitalHuazhongUniversityofScienceandTechnologyWuhan430022 DepartmentofBiochemistryTongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430022 

出 版 物：《Chinese Medical Sciences Journal》 (中国医学科学杂志（英文版）)

年 卷 期：2004年第19卷第2期

页      面：111-115页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：Supported by the National Natural Sciences Foundation of China(30170945) 

主　　题：basic fibroblast growth factor gene transfection avascular necrosis femoral head 

摘      要：Objective To explore a new method for the therapy of avascular necrosis of the femoral head. Method The recombinant plasmid pCD-rbFGF was mixed with collagen and was implanted in the necrotic femoral head. Expression of basic fibroblast growth factor (bFGF) was examined by RT-PCR and immunohistochemical method. Re-pair of the femoral head was observed by histological and histomorphometric analysis. Result Expression of bFGF was detected in the femoral head transfected with bFGF gene, indicating significant increase of angiogenesis 2 weeks after gene transfection and increased new bone formation 8 weeks after gene transfection on histom-orphometric analysis (P 0.01). Conclusion Transfection of bFGF gene enhances bone tissue angiogenesis. Repair in osteonecrosis would be accelerated accordingly.