Applications of RNA interference highthroughput screening technology in cancer biology and virology

作     者：Shan Gao Chen Yang Shan Jiang Xiao-Ning Xu Xin Lu You-Wen He Annie Cheung Hui Wang 

作者机构：Department of Oncology John Radcliffe Hospital Weatherall Institute of Molecular Medicine University of Oxford Oxford OX3 9DS UK Department of Molecular Genetics and Microbiology Stony Brook University Stony Brook NY 11794 USA Department of Immunology Duke University Durham NC 27708 USA Department of Immunology Chelsea & Westminster Hospital Imperial College of London 369 Fulham Road London SW10 9NH UK Nuffield Department of Clinical Medicine Ludwig Institute for Cancer Research Oxford Branch University of Oxford Old Road Campus Research Building Oxford OX3 7DQ UK Department of Pathology Queen Mary Hospital University of Hong Kong Pokfulam Road Hong Kong China Natural Environment Research Council Centre for Ecology & Hydrology Benson Lane Wallingford Oxfordshire OX10 8BB UK Department of Zoology University of Oxford South Parks Road Oxford OX1 3PS UK 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2014年第5卷第11期

页      面：805-815页

核心收录：

学科分类：071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 081705[工学-工业催化] 08[工学] 0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 0817[工学-化学工程与技术] 080502[工学-材料学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0836[工学-生物工程] 

基　　金：HW is supported by NERC (UK) 

主　　题：RNA interference (RNAi) short interferingRNA (siRNA) short hairpin RNA (shRNA) high-throughputscreening cancer virology 

摘      要：RNA interference （RNAi） is an ancient intra-cellular mechanism that regulates gene expression and cell function. Large-scale gene silencing using RNAi highthroughput screening （HTS） has opened an exciting frontier to systematically study gene function in mammalian cells. This approach enables researchers to identify gene function in a given biological context and will provide considerable novel insight. Here, we review RNAi HTS strategies and applications using case studies in cancer biology and virology.