Puerarin enhances superoxide dismutase activity and inhibits RAGE and VEGF expression in retinas of STZ-induced early diabetic rats

作     者：Fang Chen Hong-Quan Zhang Jun Zhu Kai-Yang Liu Hong Cheng Guo-Li Li Shan Xu Wei-Hong Lv Zheng-Gao Xie 

作者机构：Department of OphthalmologyClinical Medicine CollegeYangzhou University Institute of PharmacySchool of MedicineYangzhou University Department of BiochemistrySchool of MedicineYangzhou University Department of PathologySchool of MedicineYangzhou University 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2012年第5卷第11期

页      面：891-896页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100212[医学-眼科学] 10[医学] 

主　　题：Diabetic retinopathy Puerarin Oxidative stress Receptor for advanced glycation end products Vascular endothelial growth factor 

摘      要：Objective:To investigate the effects of puerarin on the activity of superoxide dismutase(SOD), and expressions of advanced glycation end-product(AGE) receptor(RAGE) and vascular endothelial growth factor(VEGF) in retinas of streptozotocin(STZ)-induced early diabetic rats. Methods:Diabetic rat models were established by inducing diabetes via intra-peritoneal injection of *** were randomly divided into normal(control),diabetic(DM),and DM+ puerarin *** intra-gastric administration of puerarin(500 mg/kg/day for 4 weeks),levels of SOD and malondialdehyde(MDA) were determined in serum and *** and protein expression levels of RAGE and VEGF in retinas were determined by real-lime polymerase chain reaction(RT-PCR)(mRNA) and Western blot analysis(protein levels).Results:There was significantly lower SOD activity and significantly higher MDA in serum and retinas of the DM group compared with the two other groups(P0.05).After treatment with puerarin,SOD activity increased and MDA content decreased in this group(P0.05).mRNA and protein expression levels of RACE and VECF in the DM group were significantly higher than those of the other groups (P0.05),and decreased after puerarin treatment(P0.05).Conclusions:Puerarin is able to enhance SOD activity,and inhibit RAGE and VEGF expressions in retinas of STZ-induced early diabetic ruts.