Big Grain3, encoding a purine permease, regulates grain size via modulating cytokinin transport in rice

作     者：Yunhua Xiao Dapu Liu Guoxia Zhang Shaopei Gao Linchuan Liu Fan Xu Ronghui Che Yiqin Wang Hongning Tong Chengcai Chu 

作者机构：State Key Laboratory of Plant Genomics and National Center for Plant Gene Research(Beijing)Institute of Genetics and Developmental Biologythe Chinese Academy of SciencesBeijing 100101China National Key Facility for Crop Gene Resources and Genetic ImprovementInstitute of Crop Sciencesthe Chinese Academy of Agricultural SciencesBeijing 100081China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2019年第61卷第5期

页      面：581-597页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：supported by National Natural Science Foundation of China (Nos. 91735302  31722037  91435106  91335203) 

主　　题：Grain size is an important underlying molecular local allocation 

摘      要：Grain size is an important agronomic trait affecting grain yield,but the underlying molecular mechanisms remain to be ***,we isolated a dominant mutant,big grainy(bg3-D),which exhibits a remarkable increase of grain size caused by activation of the PURINE PERMEASE gene,***3/OSPUP4 is predominantly expressed in vascular tissues and is specifically suppressed by exogenous cytokinin *** profiling revealed that the distribution of different cytokinin forms,in roots and shoots of the bg3-D mutant,is *** reverse transcription-PCR(qRT-PCR)analysis indicated that expression of rice cytokinin type-A RESPONSE REGULATOR(OsRR)genes is enhanced in the roots of the bgj-D *** results suggest that OSPUP4 might contribute to the long-distance transport of cytokinin,by reinforcing cytokinin loading into vascular bundle ***,plants overexpressing OsPUP7,the closest homolog of OsPUP4,also exhibited a similar phenotype to the bg3-D ***,subcellular localization demonstrated that OSPUP4 was localized on the plasma membrane,whereas OSPUP7 was localized to the endoplasmic *** on these findings,we propose that OSPUP4 and OSPUP7 function in a linear pathway to direct cytokinin cell-to-cell transport,affecting both its long-distance movement and local allocation.