用日本血吸虫部分cDNA表达质粒文库免疫小鼠产生的保护性效果

作     者：冯新港 林矫矫 朱建国 张亮 苑纯秀 蔡幼民 FENG Xin\|Gang\+*\ LIN Jiao\|Jiao\ ZHU Jian\|Guo\ ZHANG Liang\ YUAN Chun\|Xiu\ CAI You\|Min (Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, S

作者机构：中国农业科学院上海家畜寄生虫病研究所 

出 版 物：《生物工程学报》 (Chinese Journal of Biotechnology)

年 卷 期：2002年第18卷第2期

页      面：231-234页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：中国博士后科学基金 国家 86 3高技术研究发展计划项目基金资助 (No .10 2 0 7 0 4 0 3)~~ 

主　　题：日本血吸虫 部分cDNA 表达文库 免疫效果 质粒 小鼠模型 

摘      要：The cDNA fragments of interest were amplified using \%Sj\%λ ZipLox library as the templates by PCR and then cloned into a eukaryotic expression vector p\|CMV\|GH; A small number of DNA fragments inserted in the recombinants was identified by restriction cleavage, EST sequencing and bioinformatical analysis; mice were injected intramuscularly with the expression library(L\|CMV\|SjR) or sublibraries(L\|CMV\|SjR 1,L\|CMV\|SjR 2 and L\|CMV\|SjR 3), immunized mice were challenged with \%Schistosoma japonicum\% cercariae on day 35, the levels of IgG antibodies in sera from the immunized mice were detected by ELISA. The results demonstrated that a partial cDNA expression library of \%S.j,\% with～10\+5 transformants, was constructed, most of the recombinants contained the insert DNA fragments of interest, and these fragments had the features of protein\|coding sequences for \%Schistosome.\% There were no significant differences for the levels of IgG antibodies in sera from all of the immunized groups. Mice immunized with L\|CMV\|SjR, L\|CMV\|SjR 1 and L\|CMV\|SjR 2 developed significant protective effect against \%Sj\% infection compared to control mice injected with the empty plasmid, the rate of worm reduction was about 30%.