Phosphorylation of Serine 186 of bHLH Transcription Factor SPEECHLESS Promotes Stomatal Development in Arabidopsis
Phosphorylation of Serine 186 of bHLH Transcription Factor SPEECHLESS Promotes Stomatal Development in Arabidopsis作者机构：Key Laboratory of Plant Molecular Physiology Institute of Botany Chinese Academy of Sciences 20 Nanxincun Beijing 100093 China Department of Botany University of British Columbia Vancouver British Columbia V6T 1Z4 Canada
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2015年第8卷第5期
页 面：783-795页
核心收录：
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 071007[理学-遗传学] 0901[农学-作物学] 0902[农学-园艺学]
基 金：supported by grants from the National Natural Science Foundation of China program 中国科学院“百人计划”项目 a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada (F.S.)
主 题：Arabidopsis stomata development transcription factor phosphorylation
摘 要：The initiation of stomatal lineage and subsequent asymmetric divisions in Arabidopsis require the activity of the basic helix-loop-helix transcription factor SPEECHLESS （SPCH）. It has been shown that SPCH controls entry into the stomatal lineage as a substrate either of the MITOGEN-ACTIVATED PROTEIN KINASE （MAPK） cascade or GSK3-1ike kinase BRASSlNOSTEROID INSENSITIVE 2 （BIN2）. Here we show that three serine residues of SPCH appear to be the primary phosphorylation targets of Cyclin-Dependent Kinases A;1 （CDKA;1） in vitro, and among them Serine 186 plays a crucial role in stomatal formation. Expression of an SPCH construct harboring a mutation that results in phosphorylation deficiencies on Serine 186 residue failed to rescue stomatal defects in spch null mutants. Expression of a phosphorylation-mimic mutant SPCHS186D complemented stomatal production defects in the transgenic lines harboring the targeted expression of dominant-negative CDKA;1.N146. Therefore, in addition to MAPK- and BIN2-mediated phos- phorylation on SPCH, phosphorylation at Serine 186 is positively required for SPCH function in regulating stomatal development.
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