Honey exposure stimulates wound repair of human dermal fibroblasts

作     者：Elia Ranzato Simona Martinotti Bruno Burlando 

作者机构：Dipartimento di Scienze e Innovazione Tecnologica DiSIT University of Piemonte Orientale "Amedeo Avogadro" viale T. Michel 11 15121 Alessandria Italy 

出 版 物：《Burns & Trauma》 (烧伤与创伤（英文）)

年 卷 期：2013年第1卷第1期

页      面：32-38页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Yamada Bee Farm Grant forHoneybee Research (2010).Yamada Bee Farm Grant for Honeybee Research 

主　　题：Honey wound healing interleukins dermal fibroblast 

摘      要：Honey is widely used for treating burns, ulcers and wounds, but the mechanisms of action are poorly known and the product is mainlyused as an antimicrobial. We have examined here the wound healing properties of honey on human fi broblasts, using an in vitroscratch wound healing model. Three kinds of widely used monofl oral honeys were used, viz. acacia (Robinia pseudacacia), buckwheat(Fagopyrum sp.), and manuka (Leptospermum scoparium). Data displayed an increased wound healing activity in fi broblasts, butwith diff erent effi ciency and mechanisms of action among honeys. The eff ects of acacia and buckwheat emerged in both scratchwound and chemotaxis assays, while the eff ect of manuka was signifi cant but lower. The use of inhibitors indicated on the wholean essential role of cytosolic calcium, an important role of ERK and p38, and a secondary role of PI3K. Acacia and buckwheat,but not manuka, induced signifi cant increases in the release of interleukin-4 (IL-4), IL-6, and IL-8, indicating a correlation betweeninterleukin upregulation and wound closure effi ciency. This is consistent with our previous fi ndings suggesting a higher ability ofacacia and buckwheat to activate keratinocyte reepithelialization, with respect to manuka honey. In conclusion, our data indicatethat acacia and buckwheat honeys are particularly effi cient in facilitating fi broblast wound closure activities, suggesting newtherapeutic possibilities for this natural product.