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honey vesicle-like nanoparticles protect aged liver from non-alcoholic steatohepatitis

Acta Pharmaceutica Sinica B 2024年第8期14卷 3661-3679页

作者： Baolong Liu Phuong Linh Nguyen Han Yu Xingzhi Li Huiren Wang Tram Gia Bao Nguyen Prakash Kumar Sahoo Meghna Sur Jay Reddy Sarah Sillman Stephen D.Kachman Bara Altartouri Guoqing Lu Sathish Kumar Natarajan Mahesh Pattabiraman Jiujiu Yu Department of Nutrition and Health Sciences University of Nebraska-LincolnLincolnNE 68583USA Shaanxi Key Laboratory of Molecular Biology for Agriculture College of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China Department of Nutrition Case Western Reserve UniversityClevelandOH 44106USA School of Veterinary Medicine and Biomedical Sciences University of Nebraska-LincolnLincolnNE 68583USA Department of Statistics University of Nebraska-LincolnLincolnNE 68583USA Center for Biotechnology University of Nebraska-LincolnLincolnNE 68588USA Department of Biology University of Nebraska OmahaOmahaNE 68182USA Department of Chemistry University of Nebraska-KearneyKearneyNE 68849USA

Non-alcoholic steatohepatitis(NASH),an advanced form of non-alcoholic fatty liver disease(NAFLD),has emerged as the leading cause of liver failure and related ***,no medication is specifically approved to treat NAFLD or *** we report that oral administration of honey vesiclelike nanoparticles(H-VLNs)to naturally aged mice protects the liver from NASH development.H-VLNs are dominantly taken up by Kupffer cells in the liver and suppress hepatic chronic inflammation and further development of fibrosis and nodule formation in aged *** their reported antiinflammasome function,H-VLNs are found to inhibit the transcriptional activities of C-JUN and nuclear factor-kappa B(NF-κB).MicroRNAs miR5119 and miR5108 and phenolic compound luteolin in H-VLNs are identified in suppressing both the C-JUN and NF-kB ***,oral intake of H-VLNs represents a promising new user-friendly modality to prevent the development of NASH.

关键词： honey Extracellular vesicles Exosomes Nanoparticles microRNAs Aging Inflammation Non-alcoholic steatohepatitis

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Limit Test and pH Determination of 5-Hydroxymethylfurfural in Jiulongteng honey

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Medicinal Plant 2024年第3期15卷 23-25,29页

作者： Beiqiao YIN Yanyan CHEN Lili LIU Shenggao YIN Yanxia CEN Shiman CHEN Baise People's Hospital Baise 533000China Wuzhou Vocational College Wuzhou 543003China Guangxi University of Chinese Medicine Nanning 530000China Beihai Public Inspection and Testing Center Beihai 536000China

[Objectives]To explore the effect of storage time on pH and 5-hydroxymethylfurfural content in Jiulongteng honey.[Methods]The pH of Jiulongteng honey was determined by neutralization titration with sodium hydroxide standard *** content of 5-hydroxymethylfurfural in Jiulongteng honey was determined by *** conditions:ZORBAX SB-C 18 column(250 mm×4.6 mm,5μm)from Agilent Co.,Ltd.,acetonitrile-0.1%formic acid solution(5:95)as mobile phase,flow rate of 0.8 mL/min,5-hydroxymethylfurfural detection wavelength of 284 nm,guanosine detection wavelength of 254 nm.[Results]The pH of 12 batches of Jiulongteng honey was 3.70-3.84 in the new honey stage,3.92-4.05 in the old honey stage 1,and 4.25-4.53 in the old honey stage 2;5-hydroxymethylfurfural was not detected in the new honey stage,5-hydroxymethylfurfural was detected in FM-001 in the old honey stage 1,and 5-hydroxymethylfurfural was detected in most samples in the old honey stage 2.[Conclusions]The pH and 5-hydroxymethylfurfural content of 12 batches of Jiulongteng honey met the requirements within 3 years of *** was no 5-hydroxymethylfurfural in Jiulongteng honey,but with the extension of storage time,the detection amount of 5-hydroxymethylfurfural increased significantly even if Jiulongteng honey was stored at low ***,5-hydroxymethylfurfural can be used as an important indicator of honey freshness.

关键词： Jiulongteng honey 5-hydroxymethylfurfural pH HPLC

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Potential effects of propolis and honey in COVID-19 prevention and treatment:A systematic review of in silico and clinical studies

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Journal of Integrative Medicine 2022年第2期20卷 114-125页

作者： Witoo Dilokthornsakul Ramanya Kosiyaporn Rattanamanee Wuttipongwaragon Piyameth Dilokthornsakul Center of Pharmaceutical Outcomes Research Department of Pharmacy PracticeFaculty of Pharmaceutical SciencesNaresuan UniversityMueangPhitsanulok 65000Thailand

Background:Propolis and honey have been studied as alternative treatments for patients with coronavirus disease 2019(COVID-19).However,no study has yet summarized the full body of evidence for the use of propolis and honey in COVID-19 prevention and ***:This study systematically reviews the mechanisms of propolis and honey against severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)infection and current evidence for the use of propolis and honey in COVID-19 prevention and *** strategy:A systematic search was conducted of electronic databases including Pub Med,Scopus,Science Direct,and Cochrane Library from their inceptions to April *** criteria:Studies that evaluated the effect of propolis or bee products against SARS-Co V-2 using in silico methods,clinical studies,case reports and case series were *** extraction and analysis:A standardized data extraction form was used,and data were extracted by two independent *** synthesis was used to summarize study results concerning the use of propolis or honey in COVID-19 prevention and treatment and their potential mechanisms of action against SARS-Co ***:A total of 15 studies were *** studies were in silico studies,two studies were case reports,one study was a case series,and three studies were randomized controlled trials(RCTs).In silico studies,using molecular docking methods,showed that compounds in propolis could interact with several target proteins of SARS-Co V-2,including angiotensin-converting enzyme 2,the main protease enzyme,RNA-dependent RNA polymerase,and spike *** may have a positive effect for clinical improvement in mild and moderate-to-severe COVID-19 patients,according to case reports and case *** included RCTs indicated that propolis or honey could probably improve clinical symptoms and decrease viral clearance time when they were used as adjuvant therapy to standard of ***:In silico

关键词： Propolis honey Coronavirus disease 2019 Systematic review

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honey Safety Standards and Its Impacts on China's honey Export

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Journal of Integrative Agriculture 2012年第4期11卷 684-693页

作者： WEI Guo-xue HUANG Ji-kun YANG Jun Center for Chinese Agricultural Policy Institute of Geographical Sciences and Natural Resources ResearchChinese Academy of Sciences Beijing 100101P.R.China

Food safety standard draws increasing concerns on agricultural trade throughout the *** paper aims to assess the impact of maximum residual limit standard（MRL） of chloromycetin on honey exporting from *** achieve this objective,the paper discusses the trends of China＇s honey production and export practices,analyzes changes on MRL of chloromycetin adopted by major importing countries,and use a gravity model to estimate the impact of MRL of chloromycetin on China＇s honey *** results show that despite the rapid growth of China＇s honey production,honey export has declined significantly since *** major reason of declining honey export was mainly due to the more stringent food safety standards indicated by MRL of chloromycetin imposed by importing countries on their honey imports.

关键词： food safety standards MRL chloromycetin honey export China

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Highly Effective Detection of Amitraz in honey by Using Surface-Enhanced Raman Scattering Spectroscopy Coupled with Chemometric Methods

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Chinese Journal of Chemical Physics 2019年第4期32卷 444-450,I0002页

作者： Xin-ming Nie Jing Wang Xun Wang Ya-ping Tian Si Chen Zhou-yang Long Cheng-hua Zong School of Physics and Electronic Engineering Jiangsu Normal University Xuzhou 221116 China School of Chemistry and Materials Science Jiangsu Key Laboratory of Green Synthesis for Functional Materials Jiangsu Normal University Xuzhou 221116 China Kewen College Jiangsu normal university Xuzhou 221116 China

As an effective and universal acaricide, amitraz is widely used on beehives against varroasis caused by the mite Varroa jacobsoni. Its residues in honey pose a great danger to human health. In this study, a sensitive, rapid, and environmentally friendly surface-enhanced Raman spectroscopy method (SERS) was developed for the determination of trace amount of amitraz in honey with the use of silver nanorod (AgNR) array substrate. The AgNR array substrate fabricated by an oblique angle deposition technique exhibited an excellent SERS activity with an enhancement factor of -10^7. Density function theory was employed to assign the characteristic peak of amitraz. The detection of amitraz was further explored and amitraz in honey at concentrations as low as 0.08 mg/kg can be identified. Specifically, partial least square regression analysis was employed to correlate the SERS spectra in full-wavelength with Camitraz to afford a multiple-quantitative amitraz predicting model. Preliminary results show that the predicted concentrations of amitraz in honey samples are in good agreement with their real concentrations. Compared with the conventional univariate quantitative model based on single peak’s intensity, the proposed multiple-quantitative predicting model integrates all the characteristic peaks of amitraz, thus offering an improved detecting accuracy and anti-interference ability.

关键词： Amitraz surface-enhanced Raman spectroscopy method Oblique angle deposition honey Detection Sensing Ag nanorod Density function theory Partial least squares regression

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honey prevents hepatic damage induced by obstruction of the common bile duct

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World Journal of Gastroenterology 2008年第23期14卷 3729-3732页

作者： B Imge Erguder Sibel S Kilicoglu Mehmet Namuslu Bulent Kilicoglu Erdinc Devrim Kemal Kismet Ilker Durak Department of Biochemistry Ankara University Faculty of MedicineAnkara 06100Turkey Department of Histology and Embriology Ufuk University Faculty of MedicineAnkara 06800Turkey Ankara Research and Training Hospital Clinics of General SurgeryAnkara 06100Turkey

AIM:To examine the possible effects of honey supplementation on hepatic damage due to obstruction of the common bile duct in an experimental rat model. METHODS:The study was performed with 30 male rats divided into three groups:a sham group,an obstructive jaundice group,and an obstructive jaundice plus honey group. At the end of the study period,the animals were sacrificed,and levels of nitric oxide(NO) ,and NO synthase(NOS) activities were measured in liver tissues,and levels of adenosine deaminase(ADA) and alanine transaminase(ALT) activities were measured in serum. RESULTS:Blood ALT and ADA activities were significantly elevated in the jaundice group as compared to those of the sham group. In the obstructive jaundice plus honey group,blood ALT and ADA activities were significantly decreased as compared to those of the jaundice group. In erythrocytes and liver tissues,NO levels were found to be significantly higher in the obstructive jaundice plus honey group compared to those of the sham group. Additionally,NO levels were found to be significantly higher in liver tissues from the animals in the obstructive jaundice plus honey group than those of the jaundice ***:honey was found to be beneficial in the prevention of hepatic damage due to obstruction of the common bile duct.

关键词： honey Obstructive jaundice Adenosine deaminase Nitric oxide

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Comparative susceptibility and immune responses of Asian and European honey bees to the American foulbrood pathogen, Paenibacillus larvae

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Insect Science 2019年第5期26卷 831-842页

作者： Sasiprapa Krongdang Jay D.Evans Yanping Chen Wannapha Mookhploy Panuwan Chantawannakul 1Bee Protection Laboratory Department of BiologyFaculty of ScienceChiang Mai UniversityChiang MaiThailand Interdisciplinary Program in Biotechnology Graduate SchoolChiang Mai UniversityChiang MaiThailand USDA-ARS Bee Research LaboratoryBeltsvilleMarylandUSA Environmental Science Research Center Faculty of ScienceChiang Mai UniversityChiang MaiThailand

American foulbrood (AFB) disease is caused by Paenibacillus larvae. Currently, this pathogen is widespread in the European honey bee— Apis mellifera. However, little is known about infectivity and pathogenicity of P. lan'ae in the Asiatic cavity-nesting honey bees, Apis cerana. Moreover, comparative knowledge of P. larvae infectivity and pathogenicity between both honey bee species is scarce. In this study, we examined susceptibility, larval mortality, survival rate and expression of genes encoding antimicrobial peptides (AMPs) including defensin, apidaecin, abaecin, and hymenoptaecin in A. mellifera and A. cerana when infected with P. larvae. Our results showed similar effects of P. larvae on the survival rate and patterns of AMP gene expression in both honey bee species when bee larvae are infected with spores at the median lethal concentration (LC5 0 ) for A. mellifera. All AMPs of infected bee larvae showed significant upregulation compared with noninfected bee larvae in both honey bee species. However, larvae of A. cerana were more susceptible than A. mellifera when the same larval ages and spore concentration of P. larvae were used. It also appears that A. cerana showed higher levels of AMP expression than A. mellifera. This research provides the first evidence of survival rate, LC50 and immune response profiles of Asian honey bees, A. cerana, when infected by P. larvae in comparison with the European honey bee, A. mellifera.

关键词： Apis cerana Apis mellifera honey bee immune gene Paenibacillus larvae susceptibility

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Determination of Tetracyclines in honey Using Liquid Chromatography with Ultraviolet Absorbance Detection and Residue Confirmation by Mass Spectrometry

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Chinese Journal of Chemistry 2007年第9期25卷 1294-1299页

作者：刘艳徐锦忠丁涛李公海 Food Laboratory Jiangsu Entry-Exit Inspection and Quarantine Bureau Nanjing Jiangsu 210001 China

A determination method has been optimized and validated for the simultaneous analysis of tetracycline （TC）, oxytetracycline （OTC）, chlortetracycline （CTC） and doxycycline （DC） in honey. Tetracyclines （TCs） were removed from honey samples by chelation with metal ions bound to small Chelating Sepharose Fast Flow columns and eluted with Na2EDTA-Mcllvaine pH 4.0 buffers. Extracts were further cleaned up by Oasis HLB solid-phase extraction （SPE）, while other solid-phase extraction cartridges were compared. Chromatographic separation was achieved using a polar end-capped C 18 column with an isocratic mobile phase consisting of oxalic acid, acetonitrile and methanol. LC with ultraviolet absorbance at 355 nm resulted in the quantitation of all four tetracycline residues from honey samples fortified at 15, 50, and 100 ng/g, with liner ranges for tetracyclines of 0.05 to 2 μg/mL. Mean recoveries for tetracyclines were greater than 50% with R.S.D. values less than 10% （n= 18）. Detection limits of 5, 5, 10, 10 ng/g for oxytetracycline, tetracycline, chlortetracycline and doxycycline, respectively and quantitation limits of 15 ng/g for all the four tetracyclines were determined. Direct confirmation of the four residues in honey （2-50 ng/g） was realized by liquid chromatography-tandem mass spectrometry （LC/MS/MS）. The linear ranges of tetracyclines determined by LC/MS/MS were between 5 to 300 ng/mL, with the linear correlation coefficient r〉 0.995. The limits of detection of 1 to 2 ng/g were obtained for the analysis of the TCs in honey.

关键词： tetracyclines honey metal chelate affinity chromatography LC/MS/MS

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Preparation of Polyclonal Antibody and Development of a Biotin-streptavidin-based ELISA Method for Detecting Kanamycin in Milk and honey

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Chemical Research in Chinese Universities 2017年第6期33卷 876-881页

作者： SU Ping CHEN Xiaonan HE Zhangjing YANG Yi Beijing Key Laboratory of Environmentally Harmful Chemical Analysis College of Science Beijing University of Chemical Technology Beijing 100029 P. R. China

Kanamycin is an aminoglycoside antibiotic used increasingly in human and veterinary medicine. However, kanamycin residues in food can cause serious side effects, Here we reported the preparation of polyclonal antibody and the development of an indirect competitive biotin-streptavidin-amplified-based enzyme-linked immunosorbent assay（BA-ELISA） that can sensitively and specifically detect kanamycin residues in milk and honey. The immtmogen and coating antigen were synthesized by covalently linking kanamycin to carrier proteins using the carbodiimide method. The anti-kanamycin polyclonal antibodies were obtained from immunized rabbits. The key assay parameters were investigated and optimized. The results show that under optimum conditions, the limit of detection for kanamycin is 0.07 ng/mL and the ICs0 is 6.48 ng/mL. Cross-reactivity values of the antibody with four kanamycin analogues are all 〈1%. Trace amounts of kanamycin in milk and honey samples can be detected by this novel BA-ELISA method successfully with satisfactory recoveries of 91.0%-103.3%. The developed protocol was also validated against liquid chromatography-mass spectrometry, returning a significant correlation. These results indicate that BA-ELISA is a viable option for monitoring kanamycin residues in milk and honey.

关键词： Kanamycin Biotin-streptavidin-amplified system Enzyme-linked immtmosorbent assay(ELISA) Milk honey

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Investigation of Breaking Efficiency of N-Glycosidic Bond Between Sulfonamide and honey

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Chemical Research in Chinese Universities 2012年第2期28卷 195-199页

作者： WANG Yan-hua LI Li CHEN Chia-hung CHENG Shuenn-ren LEE Mu-sheng YU Pu-ping CHANG-CHIEN Guo-ping College of Biology and Enviornmental Engineering Zhejiang Shuren University Hangzhou 310015 P. R. China Super Micro Mass Research ＆ Technology Center Department of Business Administration Department of Mechanical Engineering Cheng Shiu University Kaohsiung 833 Taiwan P. R. China Environmental Protection Bureau Kaohsiung City Government Keelung 20143 Taiwan P. R. China

Sulfonamide residue in honey existed in a form bonded to sugar via the N-glycosidic *** would result in the possible underestimation of concentration of sulfonamide if it is not decomposed by chemical ***,in China's Mainland and Taiwan（***）,the regulation for sulfonamide residue analysis does not include hydrolysis and has been applied to a very broad range of samples,for example,egg,milk,meat,seafood as well as *** paper demonstrates the necessity of hydrolysis of it prior to extracting *** breaking efficiencies of N-glycosidic bond were investigated by 2 mol/L hydrochloric acid,pure methanol and 0.5 mol/L hydrochloric acid in methanol,*** was found that acid plays the key role in breaking the N-glycosidic bond,and it was also noticed that the dissolution of liberated sulfonamide in methanol could carry the reaction forward in favor of breaking the N-glycosidic bond.

关键词： Sulfonamide honey Liquid chromatography-electrospray ionization tandem mass spectrometry(LC-ESIMS/MS) N-Glycosidic bond

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