Defining the Mitochondrial Stress Response in Arabidopsis thaliana

作     者：Olivier Van Aken Botao Zhang Chris Carrie Vindya Uggalla Ellen Paynter Estelle Giraud James Whelan 

作者机构：ARC Centre of Excellence in Plant Energy Biology MCS Building M316 University of Western Australia 35 Stirling Highway Crawley 6009 Western AustraliaAustralia 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2009年第2卷第6期

页      面：1310-1324页

核心收录：

学科分类：090603[农学-临床兽医学] 0710[理学-生物学] 07[理学] 09[农学] 0906[农学-兽医学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：research funding from the Australian Research Council Centre of Excellence in Plant Energy Biology 

主　　题：enzymes genes green fluorescent protein heat shock proteins mitochondria oxidoreductases plant proteins plastids stress stress response Arabidopsis thaliana Genes Heat Shock Proteins Plastid stress response Arabidopsis thaliana Oxidoreductases Plant Proteins Proteins Enzymes Mitochondria Green Fluorescent Proteins 

摘      要：To obtain a global overview of how mitochondria respond to stress, we aimed to define the plant mitochondrial stress response （MSR）. By combining a set of 1196 Arabidopsis thaliana genes that putatively encode mitochondrial proteins with 16 microarray experiments on stress-related conditions, 45 nuclear encoded genes were defined as widely stress-responsive. Using green fluorescent protein （GFP） fusion assays, the mitochondrial targeting of a large number of these proteins was tested, confirming in total 26 proteins as mitochondrially targeted. Several of these proteins were observed to be dual targeted to mitochondria and plastids, including the small heat shock proteins sHSP23.5 and sHSP23.6. In addition to the well defined stress components of mitochondria, such as alternative oxidases, nicotinamide adenine dinudeotide （NAD（P）H） dehydrogenases, and heat shock proteins, a variety of other proteins, many with unknown function, were identified. The mitochondrial carrier protein family was over-represented in the stress-responsive genes, suggesting that stress induces altered needs for metabolite transport across the mitochondrial inner membrane. Although the genes encoding many of these proteins contain common cis-acting regulatory elements, it was apparent that a number of distinct regulatory processes or signals likely triggered the MSR. Therefore, these genes provide new model systems to study mitochondrial retrograde regulation, in addition to the widely used alternative oxidase model. Additionally, as changes in proteins responsive to stress did not correlate well with changes at a transcript level, it suggests that post-transcriptional mechanisms also play an important role in defining the MSR.