Mutation of the Arabidopsis Calmodulin-Like Protein CML37 Deregulates the Jasmonate Pathway and Enhances Susceptibility to Herbivory
Mutation of the Arabidopsis Calmodulin-Like Protein CML37 Deregulates the Jasmonate Pathway and Enhances Susceptibility to Herbivory作者机构：Department of Bioorganic Chemistry Max Planck Institute for Chemical Ecology Hans-Knoll-StraBe 8 07745 Jena Germany Department of Biochemistry Max Planck Institute for Chemica Eco ogy Hans-Knoll-StraBe 8 07745 Jena Germany Department of Plant Biology University of Illinois Urbana IL 61801 USA Department of Biology Queen's University Kingston Ontario Canada Canada K7L 3N6
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2014年第7卷第12期
页 面：1712-1726页
核心收录：
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0901[农学-作物学] 0902[农学-园艺学] 090201[农学-果树学]
基 金：Max-Planck-Gesellschaft MPG
主 题：herbivory oral secretion jasmonates cytosolic calcium calmodulin-like proteins.
摘 要：Throughout their life, plants are challenged by various abiotic and biotic stress factors. Among those are attacks from herbivorous insects. The molecular mechanisms underlying the detection of herbivores and the subsequent signal transduction are not well understood. As a second messenger, fluxes in intracellular Ca2＋ levels play a key role in mediating stress response pathways. Ca2＋ signals are decoded by Ca2＋ sensor proteins such as calmodulin-like proteins （CMLs）. Here, we demonstrate that recombinant CML37 behaves like a Ca2＋ sensor in vitro and, in Arabidopsis, AtCML37 is induced by mechanical wounding as well as by infestation with larvae of the generalist lepidopteran herbivore Spodoptera littoralis. Loss of function of CML37 led to a better feeding performance of larvae suggesting that CML37 is a positive defense regulator. No herbivory-induced changes in secondary metabolites such as glucosinolates or flavonoids were detected in cml37 plants, although a significant reduction in the accumulation of jasmonates was observed, due to reduced expression of JAR1 mRNA and cellular enzyme activity. Consequently, the expression of jasmonate-responsive genes was reduced as well. Summarizing, our results suggest that the Ca2＋ sensor protein, CML37, functions as a positive regulator in Ca2＋ signaling during herbivory, connecting Ca2＋ and jasmonate signaling.
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