Methods and Effects of Hongshan Cock Spermatozoa Cryopreservation

作     者：LIU Jianzhong ZHANG Yiling 

作者机构：College of Chemical Engineering and Technology Wuhan University of Science and Technology Wuhan 430081 Hubei China College of Animal Science and Technology Northwestern University of Science and Technology in Agriculture and Forest Yangling 712100 Shannxi China 

出 版 物：《Wuhan University Journal of Natural Sciences》 (武汉大学学报（自然科学英文版）)

年 卷 期：2006年第11卷第2期

页      面：447-450页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 090501[农学-动物遗传育种与繁殖] 

基　　金：SupportedbytheNationalHighTechnologyDevelopmentProgramofChina(2001AA213081) 

主　　题：Hongshan cock ,spermatozoa cryopreservation fertilities 

摘      要：The survival rates, acrosomic integrity rates of frozen semen from Hongshan cock and fertilities of the frozen-thawed spermatozoa in both pellets and straws were studied. The result shows that the average survival rate of spermatozoa in straws was 0, 451±0, 056, and 0. 390±0. 040 in pellets, the former was significantly higher than the later （n=20, p〈0, 01）, The average acrosomic integrity rate of the sperms was 0, 613±0. 049 in straws, and 0. 476±0. 057 in pellets. The former was significantly higher than the later （n= 20, p〈0.05）. The fertility rates （%） of sperms in pellets, straws and freshly colleered semen at the third day after artificial insemination （AI） were 85.32±2. 32, 87. 73±1.00 and 90.77±1.68, respectively. The fertility rate,s of the three types of semen at the fourth and fifth days were lower than the third day＇s. Variance analysts shows that the fertilities of the spermatozoa in pellets and straws were significantly lower than freshly collected semen （n=3, p〈0.01）, and the fertilities of the spermatozoa in straws were significantly higher than those in pellets （n= 3, p〈0.01）.