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Methods and Effects of Hongshan Cock Spermatozoa Cryopreservation

Wuhan University Journal of Natural Sciences 2006年第2期11卷 447-450页

作者： LIU Jianzhong ZHANG Yiling College of Chemical Engineering and Technology Wuhan University of Science and Technology Wuhan 430081 Hubei China College of Animal Science and Technology Northwestern University of Science and Technology in Agriculture and Forest Yangling 712100 Shannxi China

The survival rates, acrosomic integrity rates of frozen semen from Hongshan cock and fertilities of the frozen-thawed spermatozoa in both pellets and straws were studied. The result shows that the average survival rate of spermatozoa in straws was 0, 451±0, 056, and 0. 390±0. 040 in pellets, the former was significantly higher than the later （n=20, p〈0, 01）, The average acrosomic integrity rate of the sperms was 0, 613±0. 049 in straws, and 0. 476±0. 057 in pellets. The former was significantly higher than the later （n= 20, p〈0.05）. The fertility rates （%） of sperms in pellets, straws and freshly colleered semen at the third day after artificial insemination （AI） were 85.32±2. 32, 87. 73±1.00 and 90.77±1.68, respectively. The fertility rate,s of the three types of semen at the fourth and fifth days were lower than the third day＇s. Variance analysts shows that the fertilities of the spermatozoa in pellets and straws were significantly lower than freshly collected semen （n=3, p〈0.01）, and the fertilities of the spermatozoa in straws were significantly higher than those in pellets （n= 3, p〈0.01）.

关键词： Hongshan cock ,spermatozoa cryopreservation fertilities

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