Ultrastructural observation of tannic acid-captured exocytotic secretory granules in the anterior pituitary gland cells of the rat

作     者：林英华 刘莹莹 张旭 鞠躬 

出 版 物：《Journal of Medical Colleges of PLA(China)》 (中国人民解放军军医大学学报（英文版）)

年 卷 期：1996年第11卷第3期

页      面：217-219页

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 10[医学] 

主　　题：tannic acid exocytosis electron microscopy lactotroph corticotroph 

摘      要：Tannic acid has been employed to capture the exocytotic secretory granules by binding amino and carboxyl groups of secreted proteins and mordanting them into eletrondense material, and making them easily observed and precisely quantified by electron microscopy. The present study, using tannic acid-captured exocytotic method,aims to investigate ultrastructurally the secretion of the anterior pituitary gland cells of the rat. The exocytosis is frequently identified on lactotrophs and corticotrophs, but rarely somatotrophs,gonadotrophs and thyrotrophs. 12 lactotrophs and 12 corticotrophs of the anterior pituitary are randomly selected from each sample and the exocytotic secretory granules (ESG) are counted. The ESG(x) is 1. 1 6?. 1 6 in lactotrophs and 1. 21?0.24 in corticotrophs (P0. 05). The results morphologically suggest that the lactotrophs,as well as corticotrophs strongly respond to the stress.