Study on Population Genetic Characteristics of Qinchuan Cows Using Microsatellite Markers
秦川母牛群体遗传特性的微卫星标记研究(英文)作者机构：西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室 徐州师范大学细胞与分子生物学研究所徐州221116 河南科技大学动物科技学院
出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)
年 卷 期：2007年第34卷第1期
页 面：17-25页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 0905[农学-畜牧学] 09[农学] 071007[理学-遗传学] 090501[农学-动物遗传育种与繁殖]
基 金：This work was supported by National 863 Project of China (No. 2003AA243051) National Natural Science Foundation of China (No. 30471238) and Top-notch Personnel Foundation of Northwest A&F University
主 题：Qinchuan cattle microsatellite DNA polymorphism
摘 要：To evaluate the genetic polymorphisms and to search for available molecular markers for Qinchuan cattle, 90 Qinchuan cows were genotyped with 12 microsatellite markers. A total of 247 alleles were detected, with the number of alleles ranging from 13 （INRA005） to 33 （HEL13）, giving a mean number of 21 alleles per locus. The total and mean effective allele number were 142.6229 and 11.8852, respectively. Mean sampling variance of the allele frequency was 2.6036 × 10^-4. Allele size ranges of the 12 microsatellite loci were different. The observed heterozygosity and expected heterozygosity were from 0.7842 （INRA005） to 0.9775 （BM315） and 0.7952 （BM315） to 0.9446 （HEL13）, respectively. Mean observed heterozygosity and mean expected heterozygosity were 0.9117 and 0.9047, respectively. Polymorphism information content values were from 0.7653 （INRA005） to 0.9420 （HEL13）, and mean polymorphism information content of the 12 microsatellite loci was 0.8965. All the 12 microsatellite loci were highly polymorphic, which showed that there were rich genetic polymorphisms at these detected microsatellite loci in Qinchuan cows. At the 12 microsatellite loci, the mean fixation index was -0.0076, reflecting that the degree of heterozygote defect at these loci was not high and deviations from Hardy-Weinberg equilibrium were not significant.
维普期刊数据库
同方期刊数据库