SDS-PAGE analysis of whole cell protein and outer memrbane protein patterns of clinical isolates of Burkholderia pseudomallei

作     者：Aoichart Nontprasert Cheeraratana Cheeramakara Sasithon Pukrittayakamee David AB Dance Ty L Pitt Michael D Smith Sirivan Vanijanonta Nicholas J White 

作者机构：Faculty of Tropical MedicineMahidol UniversityBangkokThailand Health Protection AgencySouth West Tamar Science ParkDevonUnited Kingdom Public Health Laboratory ServiceCentral Public Health LaboratoryDivision of Hospital InfectionLondonUnited Kingdom Nuffield Department of MedicineJohn Radcliffe HospitalOxford UniversityUnited Kingdom 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2009年第2卷第5期

页      面：14-19页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported by the Mahidol-Oxford Tropical Medicine Research Unit UK 

主　　题：SDS-PAGE Burkholderia pseudomallei Whole cell protein Outer membrane protein Melioidosis 

摘      要：Objective:To investigate the banding patterns of whole cell protein(WCP) and outer membrane protein (OMP) of Burkholderia pseudomallei(***) in clinical isolates from patients with melioidosis. Methods:WCP and OMP of of *** in 50 clinical isolates,from 47 patients with melioidosis were prepared and separated by polyacrylamide gel electrophoresis(SDS-PAGE) using 10%gels and stained with Coomassie brilliant *** banding patterns were compared by using a laser densitometer and dendrogram. Results:There were 6 different banding patterns of WCP and 2 types of *** 1 -5 WCP had 8 common protein bands at 19.0 - 45.0 kDa with identical OMP *** banding patterns of WCP in type 6 were distinct from the others and also its OMP *** majority of clinical isolates(37/50,74%) were in type 1 *** the remaining isolates,8 were in type 2,2 in type 3,and one each was in type 4 to *** was no significant association between the WCP typing and the demographic or clinical features of the investigated ***:Despite the wide variation of clinical features of melioidosis,the results of this study show that *** had a few differences in the WCP and OMP *** typing of WCP and OMP,using SDS-PAGE analysis,could be an alternative method for phenotypic differentiation in clinical isolates of ***.