Inflammatory mediator release by Brugia malayi from macrophages of susceptible host Mastomys coucha and THP-1 and RAW 264.7 cell lines

作     者：Shiv Kumar Verma Vikas Kushwaha Vijaya Dubey Kirti Saxena Aakanksha Sharma Puvvada Kalpana Murthy 

作者机构：Division of ParasitologyCentral Drug Research InstituteCSIRLucknow 226001India 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2011年第4卷第2期

页      面：92-96页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by a grant Indian Council of Medical Research, New Delhi and SP/SO/B-46/2000 from the Department of Science and Technology,New Delhi UGC Senior Research Fellowship support to SKV 

主　　题：Cytokines Macrophage ThP-1 RAW 264.7 Brugia malayi Infective larva Microfilaria Adult worm Mastomys coucha 

摘      要：Objective:To investigate which life stage of the parasite has the ability to stimulate release of pro- or anti-inflammatory mediators from ***:The human macrophage/ monocyte cell line THP-1,the mouse macrophage cell line RAW 264.7 and naive peritoneal macrophages（PM） from the rodent host Mastomys coucha（***） were incubated at 37℃in 5%CO2 atmosphere with extracts of microfilariae（Mf）,third stage infective larvae（L3） and adult worms（Ad） of Brugia *** 48 hr post exposure,IL-1β,IL-6,TNF-α,IL-10 and nitric oxide（NO） in cell-free supernatants were ***:Extracts of all the life stages of the parasite were capable of stimulating pro-（IL-1β,IL-6 and TNF-α） and anti-inflammatory （IL-10） cytokines in both the cell lines and peritoneal macrophages of *** was the strongest stimulator of pro-inflammatory cytokines followed by L3 and Ad;however,Ad was a strong stimulator of IL-10 *** was found to have potential to modulate LPS-induced NO release in RAW ***-induced NO release was concentration dependent with maximum at 20μg/mL in both RAW and ***:The results show that parasites at all life stages were capable of stimulating pro-（IL-1β,IL-6 and TNF-α） and anti-inflammatory（IL-10） cytokines and NO release from macrophages of susceptible host ***,human and mouse macrophage cell *** can suppress the LPS-induced NO release in RAW *** findings also show that the two cell lines may provide a convenient in vitro system for assaying parasite-induced inflammatory mediator release.