miRNA-711-SP1-collagen-I pathway is involved in the anti-fibrotic effect of pioglitazone in myocardial infarction

作     者：ZHAO Na YU HaiYi YU HaiTao SUN Min ZHANG YouYi XU Ming GAO Wei 

作者机构：Department of CardiologyPeking University Third Hospital and Key Laboratory of Cardiovascular Molecular Biology and Regulatory PeptidesMinistry of HealthKey Laboratory of Molecular Cardiovascular SciencesMinistry of Education 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2013年第56卷第5期

页      面：431-439页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China (81100164,31271212,81070196,81030001) the Research Fund for the Doctoral Program of Higher Education (20100001110101,20110001120015) the Program for New Century Excellent Talents in University,the Beijing Talents Foundation 

主　　题：心肌纤维化 吡格列酮 Ⅰ型胶原 心肌梗死 心脏成纤维细胞 血管紧张素Ⅱ miRNA 小分子RNA 

摘      要：Although microRNAs(miRNAs) have been intensively studied in cardiac fibrosis,their roles in drug-mediated anti-fibrotic therapy are still ***,Pioglitazone attenuated cardiac fibrosis and increased miR-711 *** aimed to explore the role and mechanism of miR-711 in pioglitazone-treated myocardial infarction in *** results showed that pioglitazone significantly reduced collagen-I levels and increased miR-711 expression in myocardial infarction *** increased the expression of miR-711 in cardiac fibroblasts,and overexpression of miR-711 suppressed collagen-I levels in angiotensin II(Ang II)-treated or untreated *** with antagomir-711 correspondingly abolished the pioglitazone-induced reduction in collagen-I *** analysis identified SP1,which directly promotes collagen-I synthesis,as the putative target of *** was confirmed by luciferase assay and western blot ***,increased SP1 expression was attenuated by pioglitazone in myocardial infarction ***,transfection of antagomir-711 attenuated pioglitazone-reduced SP1 expression in cardiac fibroblasts with or without Ang II *** conclude that pioglitazone up-regulated miR-711 to reduce collagen-I levels in rats with myocardial *** miR-711-SP1-collagen-I pathway may be involved in the anti-fibrotic effects of *** findings may provide new strategies for miRNA-based anti-fibrotic drug research.