Two-photon spectroscopic behaviors and photodynamic effect on the BEL-7402 cancer cells of the new chlorophyll photosensitizer

作     者：ZHAO PeiDe1,2,CHEN Ping1,TANG GuoQing1,LIU JinWei2,LIN Lie1,GUO Peng1,YU Qing2, YAO JianZhong3,MA DongMing4,ZHANG GuiLan1 & CHEN WenJu1 1 Key Laboratory of Optoelectronic Information Science and Technology,Ministry of Education,Institute of Modern Optics,Nankai University,Tianjin 300071,China 2 Department of Physics,Hebei University of Technology,Tianjin 300130,China 3 College of Pharmacy,Second Military Medical University,Shanghai 200433,China 4 Tianjin College of the Traditional Chinese Medicine,Tianjin 300193,China 

作者机构：Key Laboratory of Optoelectronic Information Science and Technology Ministry of Education Institute of Modern Optics Nankai University Tianjin China Department of Physics Hebei University of Technology Tianjin China College of Pharmacy Second Military Medical University Shanghai China Tianjin College of the Traditional Chinese Medicine Tianjin China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2008年第51卷第6期

页      面：529-538页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the Science and Technology Committee of Tianjin,China(Grant No. 023107611) the Key Subject Construction Project of Hebei Provincial University the Creative Funds of Nankai University and the Fund of Hebei University of Technology 

主　　题：photosensitizer chlorophyll derivate two-photon fluorescence two-photon absorption cross-section TPPDT BEL-7402 cell 

摘      要：The spectroscopic properties of a new chlorophyll derivate photosensitizer (CDP) are studied under the excitation wavelengths at 800 and 400 nm using femtosecond pulses from a Ti: sapphire laser. The damaging effect of CDP on the BEL-7402 cancer cells is also investigated upon two-photon illumination at 800 nm. The normalized fluorescence spectra of CDP in tetrahydrofuran (THF) show that two-photon and one-photon spectra have the same distributions and the same emission bands (675 nm). The lifetimes of two- and one-photon induced fluorescence of this molecule are of the order of 5.0 ns. By comparing the data it is shown that there is some difference between the two lifetimes, but the difference is less than one nanosecond. The two-photon absorption cross section of the molecule is also measured at 800 nm and estimated as about σ _2 ≈ 31.5x10^(-50)cm^4·s·photon^(-1). The results of two-photon photodynamic therapy (TPPDT) tests show that CDP can kill all of the tested cancer cells according to the usual Eosine assessment. Our results indicate that the two-photon-induced photophysical, photochemical and photosensitizing processes of CDP may be basically similar to those of one-photon excitation. These behaviors of the sample suggest that one may find other possible methods to estimate some photosensitizers effects in details such as their distribution in cells and the reactive targets of the sub-cellular parts of some tumor cells via two-photon excitation techniques.