Development of Chitosan/Poly(L-Lactide)/Multiwalled Carbon Nanotubes Scaffolds for Bone Tissue Engineering

作     者：A. De la Paz Orozco F. Jiménez Vega S. A. Martel-Estrada A. Hernández Aguilar M. E. Mendoza-Duarte M. C. Chavarría-Gaytán C. A. Rodríguez-González I. Olivas-Armendáriz A. De la Paz Orozco;F. Jiménez Vega;S. A. Martel-Estrada;A. Hernández Aguilar;M. E. Mendoza-Duarte;M. C. Chavarría-Gaytán;C. A. Rodríguez-González;I. Olivas-Armendáriz

作者机构：Instituto de Ciencias Biomédicas Universidad Autónoma de Ciudad Juárez Chihuahua México Instituto de Arquitectura Diseno y Arte Universidad Autónoma de Ciudad Juárez Chih México Instituto de Ingeniería y Tecnología Universidad Autónoma de Cd. Juárez Chihuahua México Centro de Investigación en Materiales Avanzados Chihuahua México 

出 版 物：《Open Journal of Regenerative Medicine》 (再生医学期刊（英文）)

年 卷 期：2016年第5卷第1期

页      面：14-23页

学科分类：08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

主　　题：Chitosan Poly(L-Lactide) Multiwalled Carbon Nanotubes Mechanical Properties Biocompatibility 

摘      要：This work focuses to improve the mechanical properties and investigates the growth of osteoblasts on a degradable chitosan/poly(L-lactide)/carbon nanotubes composite for tissue engineering. The morphological and mechanical properties characterizations were performed using scanning electronic microscopy (SEM) and rheometrics analysis system (RSA). Osteoblasts differentiation was determined by alkaline phosphatase activity, total number cells by an Alamar Blue assay, cell attachment and proliferation were visualized qualitatively using SEM, mineralization was characterized by transformed infrared spectroscopy, energy dispersive spectroscopy, and X-ray diffraction. The results suggest that the chitosan/poly(L-lactide)/multiwalled carbon nanotubes composites exhibit the ability to promote cell adhesion, proliferation, and differentiation on their surface.