Optical Rotatory Dispersion Measurement of D-Glucose with Fixed Polarizer Analyzer Accessory in Conventional Spectrophotometer
Optical Rotatory Dispersion Measurement of D-Glucose with Fixed Polarizer Analyzer Accessory in Conventional Spectrophotometer作者机构：Faculty of Physics University of Regensburg Regensburg Germany.
出 版 物：《Journal of Analytical Sciences, Methods and Instrumentation》 (分析科学方法和仪器期刊（英文）)
年 卷 期：2013年第3卷第4期
页 面：234-239页
学科分类：070207[理学-光学] 07[理学] 08[工学] 0803[工学-光学工程] 0702[理学-物理学]
主 题：Optical Activity Optical Rotatory Dispersion Specific Optical Rotation Molar Optical Rotation Polarimeter Spectropolarimeter Grape Sugar D-Glucose
摘 要：In the sample compartment of a conventional spectrophotometer, mounting of a polarizer before sample and an analyzer behind sample allows the determination of the optical rotatory dispersion of optical active media by measurement of the transmission ratio of crossed and parallel arranged polarizer and analyzer. A formula for the determination of the angle of rotation is derived from the transmission ratio. The arrangement is applied to determine the molar optical rotation of D-glucose in water in the wavelength range from 220 nm to 820 nm.
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