High Levels Production of Recombinant Human Activin A—Effect upon <i>in Vivo</i>Follicle Stimulation

作     者：Nélia Ferreira de Souza Meire Coelho de Almeida Geralda Walkiria de Araujo Raquel Mariana de Melo Leite Ivan Carlos dos Santos Mercia de Paula Lima Jorge Luiz Pesquero 

作者机构：Universidade de Mogi das Cruzes Mogi das Cruzes Brazil Faculdade Pitágoras Belo Horizonte Brazil Departamento de Fisiologia e Biofísica Instituto de Ciências Biológicas Universidade Federal de Minas Gerais Belo Horizonte Brazil Departamento de Engenharia de Biossistemas Universidade Federal de São João del Rei São João del Rei Brazil Departamento de Enfermagem Básica Escola de Enfermagem Universidade Federal de Minas Gerais Belo Horizonte Brazil 

出 版 物：《Advances in Bioscience and Biotechnology》 (生命科学与技术进展（英文）)

年 卷 期：2015年第6卷第2期

页      面：96-104页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Recombinant Human Activin Yeast Superovulation 

摘      要：Activin plays an important role in numerous physiological processes such as cell differentiation and remodeling, regeneration and repair of tissues from various organs, angiogenesis, morphogenesis of glandular organs, pluripotency and differentiation of stem cells, cell adhesion and apoptosis. It participates in reproductive processes like embryogenesis, in the expression of Follicle Stimulating Hormone and Luteinizing Hormone and maturation of ovarian follicles and therefore has application in the area of reproduction of vertebrates. Given the economic importance of activin, we develop an efficient and economical method for the production of recombinant human activin A (rACT), using as expression system the yeast Pichia pastoris. rACT showed biological activity as it induced, on submicromolar dose, the increase of ovarian mass and the ovulation process in a mammal model.