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Journal of Animal Science and Biotechnology 2012年第4期3卷 188-192页

作者： Yong Yu Yunwei Pang Haichao Zhao Xiaoling Xu Zhonghong Wu Lei An Jianhui Tian Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture College of Animal Science and TechnologyChina Agricultural University

Background： Upon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor （LHCGR） is necessary for follicular maturation and ovulation, as well as luteal function. We detected mutations in the LHCGR gene and evaluated their association with Superovulation. Methods： Using polymerase chain reaction-single strand sequencing, we examined polymorphisms in LHCGR and Chinese Holstein heifers. conformation polymorphism （PCR-SSCP） and DNA the genotypes associated with Superovulation traits in 127 Results： A G/T polymorphism （ss52050737） in exon 11 was significantly associated with the total number of ova and the number of transferable embryos. Conclusions： LHCGR may be a new predictor for Superovulation in Chinese Holstein heifers.

关键词： Chinese Holstein heifer LHCGR SNP Superovulation

Relationship between heart girth, serum progesterone and Superovulation response ofdonor Holstein cows

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Asian pacific Journal of Reproduction 2019年第3期8卷 118-123页

作者： Abdel-Tawab AYKhalil Ahmed Abdel-Wahab Rabie LAbdel Aziz Department of Theriogenology Faculty of Veterinary MedicineBeni-Suef UniversityBeni-SuefEgypt Physiology Department Faculty of Veterinary MedicineMinia UniversityEl-MiniaEgypt

Objective: To determine effects of variations of heart girth, volume trait on embryo quality grade, and to explore the relationship between heart girth and circulating progesterone and correlations of circulating progesterone with embryo quality grade in superstimulated donor Holstein cows. Methods: Nineteen cows were subjected to a standard superstimulation protocol using follicle stimulating hormone. Blood samples were collected before Superovulation, at insemination and at collection of embryos for progesterone analysis. Embryo quality grades were compared between high and low heart girth donors. Moreover, Pearson's correlations were determined between heart girth, progesterone and embryo quality. Results: Variation of heart girth was not associated with significant differences in embryo quality grade between high and low heart girth donor cows (P>0.05). However, we observed a significantly higher percentage of the third-grade embryo (24.09%) in low heart girth donors compared to 9.64% in high heart girth donors. Moreover, the percentage of the transferable embryo was numerically higher and that of the degenerated embryo was numerically lower in low heart girth cows. Donor cows with low response to Superovulation (total structures ≤ 3) expressed numerically higher mean heart girth, compared to donors with high (total structures>15) and medium (total structures = 4-15) Superovulation. Heart girth and body weight of donor cows were moderately correlated (r=0.45, PSuperovulation was moderately correlated to the second-grade embryo (r=0.46, PSuperovulation;however, future studies with a higher number of cows are warranted to clarify significant inf

关键词： Heart girth Superovulation Embryo quality Progesterone Holstein cow

Effects of anestrus dog serum on Superovulation in rats and mice

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Asian pacific Journal of Reproduction 2017年第5期6卷 197-201页

作者： Hanieh Vaseghi Asghar Mogheiseh Mojtaba Kafi Masood Sepehrimanesh Mohammad Hossein Nooranizadeh Department of Clinical Sciences School of Veterinary MedicineShiraz UniversityShirazIran Gastroenterohepatology Research Center Shiraz University of Medical SciencesShirazIran

Objective:To study the effects of anestrus dog serum in rodents (contains high amount of FSH) compared with two common superovulatory programs.Methods: Rats (n=30) at diestrus phase were evenly divided into pregnant mare serum gonadotrophins (PMSG) group (administrated with 30 IU PMSG, and 48 h later following by 25 IU hCG), recombinant follicle stimulating hormone (rFSH) group (reducing dose every 12 h from 5 to 1 IU, then following by 25 IU hCG) and anestrus dog serum group (reducing dose every 12 h from 0.6 to 0.1 mL, then following by 25 IU of hCG). Mice (n=30) were also evenly divided into PMSG group (5 IU PMSG used at 13 pm, and 48 h later following by 5 IU of hCG), rFSH group (from 13 pm, reducing dose every 12 h from 2.5 to 0.5 IU, then following by 5 IU hCG) and anestrous dog serum group (from 13 pm, reducing dose every 12 h from 0.100 to 0.025 mL, then following by 5 IU hCG). Mice and rats placed with males for 24 h after last injection. Histology samples of ovaries were prepared and the numbers of corpus lutea were counted on day 14 after mating. Results: In mice, the differences among mean number of corpus lutea in all groups (P=0.01) and between FSH and dog serum group were significant (P=0.0007). But no significant differences were found between the mean number of corpus lutea in dog serum and PMSG, or between FSH and PMSG groups. In rats, mean number of corpus lutea were significant differences among three groups (P=0.01), and between PMSG and dog serum groups (P=0.02). Conclusions: Superovulatory response in anestrus dog serum group is similar to PMSG group in mice, which is relatively similar to FSH in rats.

关键词： Superovulation Anestrus dog PMSG rFSH Rodent

Increased Oocyte Production after Acupuncture Treatment during Superovulation Process in Mice

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Journal of Reproduction and Contraception 2009年第1期20卷 35-44页

作者： Chun-lan JIN Kazuo Tohya Koichi Kuribayashi Michio Kimura Yuki-hisa Hirao Instimte of Acupuncture and Moxibustion China Academy of Chinese Medical Sciences Beijing 100700 China Department of Anatomy Kansai University of Health Sciences Kumatori Osaka 5900482 Japan Department of Immunology and Pathology Kansai University of Health Sciences Kumatori Osaka 5900482 Japan

Objective To investigate whether acupuncture treatment during Superovulation process improves ovarian response and increases egg production. Methods ICR female mice aged 12 -5 weeks were divided into control group, anaesthesia group and acupuncture group. Female mice were injected intraperitoneally with pregnant mare＇s serum gonadotropin （PMSG）, followed by human chorionic gonadotropin （hCG） injection 56 h later. Anaesthesia group and acupuncture group were anaesthetized three times by injecting 10% nembutal solution according to 7.5-10.0μl/g weight. Acupuncture group was treated three times with puncture Sanyinjiao Points（SP6） under anaesthesia. After 17 h of hCG injection, eggs were recovered and ovaries were isolated. Matured eggs were counted, ovarian histology and expression of follicle stimulating hormone receptor（FSH-R） in ovary were analyzed. Results Acupuncture treatment statistically increased the number of ovulated eggs. Histological section showed that some matured follicles left in the ovaries of control and anaesthesia groups after ovulation. On the contrary, all matured follicles ruptured and converted into corpus lutea in Acupuncture group. Expression of FSH-R in ovary was decreased in acupuncture group compared with those of the two others. Conclusion Acupuncture treatment during Superovulation process improves ovarian response so as to increase egg production. The positive effect of acupuncture may be associated with regulated FSH-R expression.

关键词： acupuncture Superovulation egg production ovarian response FSH-R expression

Research on the Optimal Dose of FSH for Superovulation of Mongolian Cattle

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Agricultural Biotechnology 2014年第6期3卷 31-33,37页

作者： Lidong HAN Feng WANG Shenyuan WANG Dong ZHANG Chunhua HUANG Gang ZHONG Xianbao ZHANG Lijun ZHOU Zhichao ZHAO Yingchun XU Li ZHANG College of Life Science Inner Mongolia Agricultural University Inner Mongolia Key Laboratory of Bio-manufacturing College of Life Sciences Nankai University Inner Mongolia Livestock Improvement Station Baotou Livestock Improvement Station

In this study, purebred Mongolian cattle were treated with a series of technical means including Superovulation, artificial insemination, non-surgical embryo flushing, embryo grading and embryo cryopreservation, aiming at exploring a set of mature, stable production and breeding program for purebred Mongolian cattle embryos. The results showed that the optimal dose of FSH for Superovulation of bred Mongolian cattle was 410 IU; the optimal dose of FSH for Superovulation of muhiparous Mongolian cattle was 440 IU. Bred Mongolian cattle exhibited higher average number of available embryos and embryo utilization rate compared with muhiparous Mongolian cattle, but the latter exhibited higher average number of embryos recovered.

关键词： Mongolian cattle Superovulation Bred Multiparous FSH

EFFECTS OF DIFFERENT HORMONE COMBINATIONS ON Superovulation IN RIVER BUFFALOES

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EFFECTS OF DIFFERENT HORMONE COMBINATIONS ON SUPEROVULATION ...

Superovulation of the Cloned Cattle Derived from Somatic Cells and the Transfer of the Vitrified-Thawed Embryos of the Cloning Cattle

The 5th Annual Conference of the Asian Reproductive Biotechnology Society

作者： Guang-sheng QIN~(1,2,3) Ming-tang CHEN~2 Xian-wei LIANG~(2*) Xiu-fang ZHANG~2 Chun-ying PANG~2 Sheng-ju WEI~2 Fen-xiang HUANG~2 He-sheng JIANG~(3,3*) 1 College of Animal Science & Technology,Guangxi University,Nanning,530004,China 2 Guangxi Buffalo Research Institute,Chinese Academy of Agricultural Science,Nanning 530001,China. 3 Nanning Ovagene Biotechonology Co.,Ltd,Nanning,530003,China

This study was conducted to evaluate effects of different hormones combinations with FSH,PG,LHRH-A3 and LH on Superovulation in fiver baffaloes.Thirty-five heads of river buffaloes were divided into six groups as foll... 详细信息

关键词： River buffalo Superovulation FSH LHRH-A3

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Agricultural Sciences in China 2004年第12期3卷 937-942页

作者： DONGYa-juan BAIXue-jin LIJian-dong CHENGMing TheCenterofAnimalEmbryoEngineering LaiyangAgriculturalCollegeLaiyang265200P.R.China

In this experiment, it was designed to carry out Superovulation on the two cloned cattles, vitrification and transfer of the embryos recovered from them. First of all, it was carried out vitrification on embryos obtained by IVF. Results showed that there were no significant differences between the blastocysts (obtained by IVF) vitrified in EPS10 and these in EPS20 on the resuscitative rate and the developmental rate. The hatched rate of the blastocysts vitrified in EPS10 (31.3%, 35/112) was significantly higher than that in EPS20 (12.2%, 13/107)(PSuperovulation, six (four usable embryos) and ten (nine usable embryos) embryos were respectively recovered from Kangkang and Shuanghuang. Two embryos were selected from the recovered embryos of each cloned cattle to freeze in EPS20, subsequently thawed and transferred into luteal ipsilateral uterine horns of 4 Holstein recipient cows after synchronization of estrus, respectively. At last, one recipient cow (No. 9908) became pregnant and delivered one healthy calf (descendant of the cloned cattle-Shuangshuang). The results of this experi- ment show that the cloned cattle as well as common cattle had better response to the exotic FSH and better ability to multiovulation, the embryos recovered from the cloned cattle can be vitrificated.

关键词： Cloned cattle Superovulation Embryo Vitrification

Relationship between stem cell factor and gonadotropin in ovarian follicles development during Superovulation cycles

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Military Medical Research 2002年第3期17卷 192-195页

作者：王桂红赵海波辛晓燕林国成 Xijing hospital Fourth Military Medical University China Xi’an 710033 Department of Nuclear Medicine Department of Obstetrics and Gynecology

Objective: To study whether stem cell factor (SCF) and gonadotropin work synergisticly in super-ovulation stimulation of an in-vitro fertilization and embryo transfer (IVF-ET) program. Methods: Total cycles of 30 IVF-ET patients with regular menstrual period were studied. The same Superovulation regimen was employed. Enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) were used to determine the levels of SCF, follicular-stimulating hormone (FSH) and luteinizing hormone (LH) in follicu-lar fluid (FF) during oocyte pick-up (OPU) and in serum before and after Superovulation. Results: FF-SCF levels were significantly higher in high and moderate reactive groups [(564±64) ng · L-1, (532±55) ng · L-1, respectively] than that in low reactive group [(352±78) ng · L-1, P<0. 01]; FF-FSH levels were significantly different in three groups [(23. 7±5. 7) U · L-1, (24. 5±8. 9) U · L-1, and (15. 4±4.1) U · L-1, respectively, P<0. 05]. FF-LH levels were lower in low reactive group (33. 0±7.

关键词： stem cell factor gonadotropin Superovulation follicle follicular fluid

The Effect of GnRHa Induced Superovulation on Endometrial Morphology and Estrogen Receptor and Progesterone Receptor in Mouse

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Journal of Reproduction and Contraception 2002年第3期13卷 152-157页

作者： Yong-MiaoPAN Yi-FuSHI Women＇sHospital SchoolofMedicineZhejiangUniversityHangzhou310006China Women＇sHospital SchoolofMedicineZhejiangUniversityHangzhou310006China

To evaluate the effect of GnRHa induced Superovulation protocol on endometrial morphology and function. Material & Methods Forty ICR mice were randomly allocated into 4 groups, among them, 2 experimental groups were injected with GnRHa+HMG+hCG, another 2 groups were given saline of same volume as control group. The uterine tissues were investigated at 24 h and 48 h after administration (experimental group) or ovulation (control group).The endometrial thickness, the size of gland and glandular lumen, the total area of glandular cells, the average height of glandular epithelium were measured from routine histological slides using computerized image analysis. The SP immunohistochemistry techniques with monoclonal antibodies were employed to semi quantitatively analize the estrogen receptor (ER) and progesterone receptor (PR) in glandular cells. Results The endometrial thickness was not significantly different between experimental groups and control groups at 24 h and 48 h (P>0.05).The average area, perimeter, maximal diameter of single gland and glandular lumen, the total area, average height of glandular epithelium in experimental groups were significantly smaller than those of in control groups at equivalent time stages (all P<0.01). The asynchronous development of gland epithelium and stroma cells, namely, pesudostratified glandular epithelium and predecidual changes of stroma cells were seen at same time in experimental groups. The positive percentage (%) and expression intense of ER and PR in glandular epithelium cells were significantly lower in experimental groups than in control groups (P<0.05). Conclusion The protocol with GnRHa had a negative effect on endometrial histological structure and down regulated the express of ER and PR, suggesting that this protocol effect on the endometrial morphology and function and could not facilitate the formation of a physiologic endometrium completely, which may be one of the causes of low pregnancy rates.

关键词： GnRHa Superovulation endometrium histology estrogen Receptor (ER) progesterone receptor (PR) mice

The Effect of n-Hexane on the Gonad Toxicity of Female Mice

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Biomedical and Environmental Sciences 2012年第2期25卷 189-196页

作者： LIU Jin HUANG Hui Ling PANG Fen ZHANG Wen Chang Department of Occupational and Environmental Health School of Public Health Fujian Medical University Fuzhou 350004 Fujian China Fujian Medical University Union Hospital Fuzhou 350004 Fujian China

Objective To investigate the toxic effects of n-hexane on the Ganod of female mice.Methods n-Hexane was administered to four groups of mice by inhalation at doses of 0,3.0,15.1,and 75.8 mL/m3 respectivelyfor five weeks.Each group consisted of 10 mice,of which half were injected in first with 10 IU of pregnant mare serum gonadotrophin（PMSG） on the 33rd days,and then with 10 IU of human chorionic gonadotrophin（HCG） 48 hrs later.After the treatment,mouse sera were sampled and ovulating hormone（LH）,follicle-stimulating hormone（FSH）,estradiol（E2）,and progesterone（P4） levels were measured by electrochemiluminescence immunoassays（ECLIA）.In each group,the right ovaries of the non-super-ovulated mice were stained with hematoxylin and eosin while ovaries on the left side were prepared with the TUNEL method in order to detect apoptotic cells.Results The duration of the diestrus stage decreased significantly（P0.05） in the 75.8 mL/m3 group.All super-ovulated mice in each treatment group produced fewer eggs than those in the control group（P0.05）.The number of follicles in ovaries in the 75.8 mL/m3 group was smaller compared with the control group（P0.05）.The serum P4 levels in each treatment group were lower than those in the control group（F=6.196,P0.01）.The cell apoptotic rate in the 75.8 mL/m3 group was higher（P0.05）.Conclusion n-Hexane may have directly mediated via alterations hormone secretion and promoted granulosal cell apoptotic,which may be one of the important mechanisms for n-hexane induced mouse ovary impairment.

关键词： n-Hexane Superovulation Hormone Ovary Cell apoptotic rate