Diagnosis of Toxoplasma gondii infection in pregnant women using automated chemiluminescence and quantitative real time PCR

作     者：Ehsan Ahmadpour Elmira Zargami Mahmoud Mahami-Oskouei Adel Spotin Abbas Shahbazi Hossein Samadi Kafil Saba Rajabi Paria Alizadeh Yagoob Azadi Reza Bahaj Firouz Shahrivar Aleksandra Barac 

作者机构：Infectious and Tropical Disease Research Center Tabriz University of Medical Sciences Students Research Committee Tabriz University of Medical Sciences Department of Parasitology and Mycology Faculty of Medicine Tabriz University of Medical Sciences Drug Applied Research Center Tabriz University of Medical Sciences Clinic for Infectious and Tropical Diseases Clinical Centre of Serbia Faculty of Medicine University of Belgrade 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2019年第12卷第1期

页      面：26-31页

核心收录：

学科分类：100208[医学-临床检验诊断学] 1002[医学-临床医学] 100211[医学-妇产科学] 10[医学] 

基　　金：supported by Infectious and Tropical Disease Research Center Tabriz University of Medical Sciences Tabriz Iran(Grant No.94/2-5/17) 

主　　题：Toxoplasmosis Toxoplasma gondii Chemiluminescence immunoassay Real time PCR Pregnancy 

摘      要：Objective: To identify serodiagnosis and quantification of Toxoplasma gondii(T. gondii) infection among pregnant women in Salmas, northwest of Iran. Methods: In this crosssectional study, 276 blood samples were collected from pregnant women referred to the health care centers in Salmas city. The demographic variables were also recorded. Titers of antiToxoplasma IgM and IgG antibodies(Ab) were determined using the chemiluminescence immunoassay. Quantitative real-time PCR targeting the T. gondii repeated element gene was also performed on the blood sample. Results: Out of all, 19.92%(55/276) and 2.17%(6/276) patients were seropositive for anti-Toxoplasma IgG and IgM Ab, respectively. Moreover, the presence of T. gondii DNA was observed in 12.31%(34/276) blood samples. A significant relationship was observed between the IgG Ab seropositivity and contact with the cat as a risk factor(P=0.022). Conclusions: The seroprevalence rate of T. gondii infection in pregnant women is relatively low. Consequently, the seronegative pregnant women are at risk, and a considerable rate of positive blood samples for the presence of parasite s DNA should not be ignored. Besides, quantitative real-time PCR could be considered as an accurate method for diagnosis of acute toxoplasmosis especially when the precise results are of the most importance in pregnancy. Limiting contact with cats is also suggested for pregnant women.