3-N-Butylphthalide mitigates high glucose-induced injury to Schwann cells:association with nitrosation and apoptosis

作     者：Dan-Dan Xu Wen-Ting Li Dan Jiang Huai-Guo Wu Ming-Shan Ren Mei-Qiao Chen Yuan-Bo Wu 

作者机构：Department of Neurology First Affiliated Hospital of University of Science and Technology of China Department of Neurology Anhui Second People's Hospital Department of Infection First Affiliated Hospital of University of Science and Technology of China Department of Neurology Affiliated Anhui Provincial Hospital of Anhui Medical University 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2019年第14卷第3期

页      面：513-518页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 100215[医学-康复医学与理疗学] 10[医学] 

基　　金：supported by the Natural Science Foundation of Anhui Province,China,No.1608085MH209(to YBW) New Medicine of University of Science and Techology of China,No.WK110000036(to YBW) 

主　　题：nerve regeneration Schwann cells 3-n-butylphthalide 3-nitrotyrosine nitration stress uric acid peroxynitrite anions diabetic peripheral neuropathy apoptosis proliferation neural regeneration 

摘      要：A high glucose state readily causes peripheral axon atrophy, demyelination, loss of nerve fiber function, and delayed regeneration. However, few studies have examined whether nitration is also critical for diabetic peripheral neuropathy. Therefore, this study investigated the effects of high glucose on proliferation, apoptosis, and 3-nitrotyrosine levels of Schwann cells treated with butylphthalide. In addition, we explored potential protective mechanisms of butylphthalide on peripheral nerves. Schwann cells were cultured in vitro with high glucose then stimulated with the peroxynitrite anion inhibitors uric acid and 3-n-butylphthalide for 48 hours. Cell Counting Kit-8 and flow cytometry were used to investigate the effects of uric acid and 3-n-butylphthalide on proliferation and apoptosis of Schwann cells exposed to a high glucose environment. Effects of uric acid and 3-n-butylphthalide on levels of 3-nitrotyrosine in Schwann cells were detected by enzyme-linked immunosorbent assay. The results indicated that Schwann cells cultured in high glucose showed decreased proliferation, but increased apoptosis and intracellular 3-nitrotyrosine levels. However, intervention with uric acid or 3-n-butylphthalide could increase proliferation of Schwann cells cultured in high glucose, and inhibited apoptosis and intracellular 3-nitrotyrosine levels. According to our data, 3-n-butylphthalide may inhibit cell nitrification and apoptosis, and promote cell proliferation, thereby reducing damage to Schwann cells caused by high glucose.