Human Cytomegalovirus UL138 Open Reading Frame Is Highly Conserved in Clinical Strains

作     者：Ying Qi Rong He Yan-ping Ma Zheng-rong Sun Yao-hua Ji Qiang Ruan 

作者机构：Virus Laboratory Shengjing Hospital China Medical University Shenyang 110004 China 

出 版 物：《Chinese Medical Sciences Journal》 (中国医学科学杂志（英文版）)

年 卷 期：2009年第24卷第2期

页      面：107-111页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 07[理学] 09[农学] 071007[理学-遗传学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：Supported by the National Natural Science Foundation of China (30801254) 

主　　题：人巨细胞病毒 开放阅读框架 临床症状 菌株 PCR扩增产物 编码蛋白质 保守 聚合酶链反应 

摘      要：Objective To investigate the variability of human cytomegalovirus(HCMV) UL138 open reading frame(ORF) in clinical strains.Methods HCMV UL138 ORF was amplified by polymerase chain reaction(PCR) and PCR amplification products were sequenced directly,and the data were analyzed in 19 clinical strains.Results UL138 ORF in all 30 clinical strains was amplified successfully.Compared with that of Toledo strain,the nucleotide and amino acid sequence identities of UL138 ORF in all strains were 97.41% to 99.41% and 98.24% to 99.42%,respectively.All of the nucleotide mutations were substitutions.The spatial structure and post-translational modification sites of UL138 encoded proteins were conserved.The result of phylogenetic tree showed that HCMV UL138 sequence variations were not definitely related with different clinical symptoms.Conclusion HCMV UL138 ORF in clinical strains is high conservation,which might be helpful for UL138 encoded protein to play a role in latent infection of HCMV.