Sperm DNA fragmentation, recurrent implantation failure and recurrent miscarriage

作     者：Carol Coughlan Helen Clarke Rachel Cutting Jane Saxton Sarah Waite William Ledger Tinchiu Li Allan A Pacey 

作者机构：Department of Human Metabolism Academic Unit of Reproductive and Developmental Medicine University of Sheffield Level 4 The Jessop Wing Tree Root WalkSheffield S10 2SF UK Assisted Conception Unit Jessop Wing Tree Root Walk Sheffield S10 2SF UK Department of Obstetrics and Gynaecology School of Women'sand Children's Health University of New South Wales Royal Hospital for Women Sydney New South Wales Australia 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2015年第17卷第4期

页      面：681-685页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 1002[医学-临床医学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0905[农学-畜牧学] 09[农学] 090501[农学-动物遗传育种与繁殖] 

基　　金：supported by The University of Sheffield  Jessop Fertility and the Jessop Wing Research Executive Small Grants Scheme 

主　　题：DNA recurrent implantation failure recurrent miscarriage sperm 

摘      要：Evidence is increasing that the integrity of sperm DNA may also be related to implantation failure and recurrent miscarriage （RM）. To investigate this, the sperm DNA fragmentation in partners of 35 women with recurrent implantation failure （RIF） following in vitro fertilization, 16 women diagnosed with RM and seven recent fathers （control） were examined. Sperm were examined pre- and post-density centrifugation by the sperm chromatin dispersion （SCD） test and the terminal deoxynucleotidyl transferase dUTP nick end labeling （TUNEL） assay. There were no significant differences in the age of either partner or sperm concentration, motility or morphology between three groups. Moreover, there were no obvious differences in sperm DNA fragmentation measured by either test. However, whilst on average sperm DNA fragmentation in all groups was statistically lower in prepared sperm when measured by the SCD test, this was not seen with the results from the TUNEL assay. These results do not support the hypothesis that sperm DNA fragmentation is an important cause of RIF or RM, or that sperm DNA integrity testing has value in such patients. It also highlights significant differences between test methodologies and sperm preparation methods in interpreting the data from sperm DNA fragmentation tests.