Protective effects of lidocaine injected into the hepatoduodenal ligament on warm ischemia-reperfusion injury to the rat liver

作     者：陈明易 李崇辉 黄志强 刘巨超 周宁新 黄晓强 王燕生 

作者机构：Department of Hepatobiliary Surgery General Surgical Research Institute Chinese People' s Liberation Army General Hospital Beijing 100853 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2004年第17卷第2期

页      面：275-279页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：This project was financially supported by the Chinese People ' s Liberation Armoy General Hospital (No. C01YZ03) 

主　　题：liver ischemic preconditioning ischemia-reperfusion injury lidocaine inneruation 

摘      要：Background Ischemia-reperfusion (IR) injury to the liver is still a critical and daunting problem in the field of hepatobiliary surgery. Ischemic preconditioning (IP) of the liver serves as an effective approach against IR injury. This study was to develop a novel procedure that could mimic IP, but might be more feasible than IP during surgery. Methods Eighty-two SD rats were randomly divided into 5 groups. L group (n = 21 ): 0. 4% lidocaine (10 mg/kg) was injected into the hepatoduodenal ligament 10 minutes before a 40-minute hepatic IR. IP group (n =16): a 5-minute ischemia was followed by a 10-minute reperfusion prior to a 40-minute hepatic IR. ILR group (n =15): after a 40-minute ischemia of the liver, 0.4% lidocaine (10 mg/kg) was injected into the hepatoduodenal ligament 10 minutes prior to a 40-minute reperfusion of the liver. IR group (n =15): the liver of the rat was subjected to a 40-minute IR. Control group (n = 15): 0. 9% sodium chloride was injected into the hepatoduodenal ligament without other treatments. The levels of plasma alanine transaminase (ALT) and aspartate transaminase (AST) were determined for each group after treatment. Results The mean concentrations of ALT and AST were (379. 80 ±141. 69) U/L and (606. 05 ± 220. 26) U/L for the L group, (334. 64 ±141. 94) U/L and (625. 68 ±267. 06) U/L for the IP group, (523. 36 ±170. 35) U/L and (765. 47 ±238. 45) U/L for the ILP group, (524. 29 ±163. 59) U/L and (764. 63 ±246. 79) U/L for the IR group, and (150. 90 ±27. 05) U/L and (298. 15 ±47. 68) U/L for the control group (standard error of the mean). Conclusion A significant decrease in ALT and AST levels was observed in the L and IP groups when compared to the ILR and IR groups (P0. 05). These results suggest that pretreatment with lidocaine injected into the hepatoduodenal ligament prior to IR provide