Short-term effects of obestatin on hexose uptake and triacylglycerol breakdown in human subcutaneous adipocytes
Short-term effects of obestatin on hexose uptake and triacylglycerol breakdown in human subcutaneous adipocytes作者机构：Institut des Maladies Métaboliques et Cardiovasculaires Institut National de la Santé et de la Recherche Médicale Université Paul Sabatier Department of Pharmacy Faculty of Health Sciences Universidad San Jorge
出 版 物：《World Journal of Diabetes》 (世界糖尿病杂志（英文版）（电子版）)
年 卷 期：2018年第9卷第1期
页 面：25-32页
核心收录：
基 金：Supported by Erasmus plus Traineeship program
主 题：Insulin Lipolysis Adipokines Glucose uptake Obestatin Human adipocytes
摘 要：AIM To study complete dose-dependent effects of obestatin on lipolytic and glucose transport activities in human adipocyte preparations highly responsive to *** Adipocytes were prepared by liberase digestion from subcutaneous abdominal adipose tissue obtained from overweight subjects undergoing plastic surgery. The index of lipolytic activity was the glycerol released in the incubation medium, while glucose transport was assessed by [~3H]-2-deoxyglucose uptake *** When tested from 0.1 nmol/L to 1 μmol/L, obestatin did not stimulate glycerol release; it did not inhibit the lipolytic effect of isoprenaline and did not alter the insulin antilipolytic effect. Obestatin hardly activated glucose transport at 1 μmol/L only. Moreover, the obestatin stimulation effect was clearly lower than the threefold increase induced by insulin 100 nmol/*** Low doses of obestatin cannot directly influence lipolysis and glucose uptake in human fat cells.
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