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Effective fraction from Simiao Wan prevents hepatic insulin resistant by inhibition of lipolysis via AMPK activation

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Chinese Journal of Natural Medicines 2022年第3期20卷 161-176页

作者： JIANG Qi-Xin CHEN Yi-Meng MA Jing-Jie WANG Yu-Ping LI Ping WEN Xiao-Dong YANG Jie State Key Laboratory of Natural Medicines China Pharmaceutical UniversityNanjing 210009China

Simiao Wan(SMW)is a traditional Chinese formula,including Atractylodis Rhizoma,Achyranthis Bidentatae Radix,Phellodendri Chinensis Cortex and Coicis Semen at the ratio of 1:1:2:*** can be used to the treatment of ***,its bioactive compounds and underlying mechanism are *** study was designed to screen the antilipolytic fraction from SMW and investigate its therapeutic mechanisms on hepatic insulin *** fractions of SMW were prepared by membrane separation combined with macroporous resin and their antilipolytic activity was screened in fasted *** effects of 60%ethanol elution(ESMW)on lipolysis were investigated in 3T3-L1 adipocytes stimulated by palmitic acid(PA)and high fat diet(HFD)-fed *** our study,ESMW was the bioactive fraction responsible for the antilipolytic activity of SMW and 13 compounds were characterized from ESMW by UHPLC-QTOF-MS/*** suppressed protein kinase A(PKA)-hormone-sensitive lipase(HSL)related lipolysis and increased AMP-activated protein kinase(AMPK)phosphorylation in PA challenged 3T3-L1 *** knockdown abolished the inhibitory effects of ESMW on IL-6 and HSL pSer-660,revealing that the antilipolytic and anti-inflammatory activities of ESMW are AMPK ***,ESMW ameliorated insulin resistance and suppressed lipolysis in HFD-fed *** inhibited diacylglycerol accumulation in the liver and inhibited hepatic *** medium collected from ESMW-treated 3T3-L1 cells ameliorated insulin action on hepatic gluconeogenesis in liver cells,demonstrating that the antilipolytic activity contributed to ESMW beneficial effects on hepatic glucose *** conclusion,ESMW,as the antilipolytic fraction of SMW,inhibited PKA-HSL related lipolysis by activating AMPK,inhibiting diacylglycerol(DAG)accumulation in the liver and thereby improving insulin resistance and hepatic gluconeogenesis.

关键词： Simiao Wan Insulin resistance lipolysis AMPK

Effect of dietary docosahexaenoic acid on lipogenesis and lipolysis in black sea bream,Acanthopagrus schlegeli

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Acta Oceanologica Sinica 2007年第1期26卷 112-121页

作者： JI Hong OM Ahamd Daud YOSHIMATSU Takao UMINO Testuya NAKAGAWA Heisuke FURUHASHI Makoto SAKAMOTO Shuichi College of Animal Science and Technology Northwest Agriculture and Forestry University Shaanxi 712100 China Graduate School of Biosphere Science Hiroshima University Higashi - hiroshima 739 - 8528 Japan Marine Finfish Production and Research Center Terengganu 22200 Malaysia Farming System Division National Research Institute of Aquaculture Fisheries Research Agency Mie 516 -0913 Japan Oriental Yeast Co Ltd Chiba 281 -0002 Japan

Hatchery-roared juvenile black sea breams are characterized by a low level of highly unsaturated fatty acids in their bodies, as compared with wild fish. To assess the effect of docosahaxaenoic acid （DHA） on lipegenic and lipelysis enzymes, one-year fish were roared on a casein-based purified diet and a DHA fortified diet （ 1.5% DHA ethyl ester/kg diet） for 60 d, followed with a period of 55 d for starvation. Dietary DHA was effectively incorporated into the fish body. Fortification of DHA depressed activities of glucose-6-phosphate dehydrogenase and NADP-isocitrate dehydrogenase as lipogenic enzymes in the hepatopancroas and intraperitoneal fat body. Carnitine palmitoyltransferase as lipolysis enzyme in the hepatopancreas was active in the DHA fortified fish. Starvation after feeding experiment induced increased carnitine palmitoyltransferase activity in both control and DHA fortified fish and the activity remained higher in the DHA fortified fish, while the monoenes were selectively consumed prior to highly unsaturated fatty acids. These results indicated that dietary DHA depressed lipogenesis and activated lipolysis.

关键词： DHA enzyme lipogenesis lipolysis black sea bream

DHA induces adipocyte lipolysis through endoplasmic reticulum stress and the cAMP/PKA signaling pathway in grass carp(Ctenopharyngodon idella)

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Animal Nutrition 2023年第2期13卷 185-196页

作者： Xiaocheng Huang Chenchen Bian Hong Ji Shanghong Ji Jian Sun College of Animal Science and Technology Northwest Agriculture and Forestry UniversityYangling 712100China

Docosahexaenoic acid(DHA)is a biologically active fatty acid that reduces the accumulation of ***,the molecular mechanism underlying this process,particularly in fish,is not well *** studies show that endoplasmic reticulum(ER)stress triggers the activation of the unfolded protein response,which has been revealed to play an essential role in lipid *** this study,we explored the effect of DHA on ER stress and investigated the potential molecular mechanisms underlying DHA-induced adipocyte lipolysis in grass carp(Ctenopharyngodon idella)both in vivo and in *** found that DHA remarkably reduced the triglyceride content,increased the secretion of glycerol,pro-moted lipolysis in adipocytes and evoked ER stress,whereas inhibiting ER stress using 4-phenyl butyric acid(4-PBA)inhibited the effects of DHA(Plipolysis,demonstrating that IRE1a and X-box binding protein 1 potentially participate in DHA-induced *** also activated the cyclic adenosine monophosphate(cAMP)-dependent protein kinase A(PKA)pathway by increasing the level of cAMP and activating the PKA enzyme(Plipolysis by inhibiting the cAMP/PKA signaling ***,inhibiting ER stress us-ing 4-PBA also inhibited lipolysis and alleviated DHA-induced activation of the cAMP/PKA signaling pathway,suggesting that ER stress may participate in DHA-induced lipolysis through the activation of the cAMP/PKA signaling *** data illustrate that DHA supplementation can be a promising nutritional strategy for ameliorating lipid accumulation in grass *** present study elucidated the molecular mechanism for DHA-induced lipolysis in grass carp adipocytes and emphasized the impor-tance of ER st

关键词： Adipocyte cAMP/PKA pathway Docosahexaenoic acid Endoplasmic reticulum stress Grass carp lipolysis

Gastrointestinal lipolysis and trans-epithelial transport of SMEDDS via oral route

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Acta Pharmaceutica Sinica B 2021年第4期11卷 1010-1020页

作者： Fei Xia Zhongjian Chen Quangang Zhu Jianping Qi Xiaochun Dong Weili Zhao Wei Wu Yi Lu Key Laboratory of Smart Drug Delivery of MOE School of PharmacyFudan UniversityShanghai 201203China Shanghai Skin Disease Hospital Tongji University School of MedicineShanghai 200443China

Self-microemulsifying drug delivery systems(SMEDDSs)have recently returned to the limelight of academia and industry due to their enormous potential in oral delivery of ***,information on gastrointestinal lipolysis and trans-epithelial transport of SMEDDS is ***-caused quenching(ACQ)fuorescent probes are utilized to visualize the in vivo behaviors of SMEDDSs,because the released probes during lipolysis are quenched upon contacting *** SMEDDSs composed of medium chain triglyceride and different ratios of Tween-80 and PEG-400 are set as models,meanwhile Neoral?was used as a *** SMEDDS droplets reside in the digestive tract for as long as 24 h and obey frst order kinetic law of *** increased chain length of the triglyceride decreases the lipolysis of the *** vivo imaging of main tissues and histological examination confrm the trans-epithelial transportation of the SMEDDS *** 2%-4%of the given SMEDDSs are transported via the lymph route following epithelial uptake,while liver is the main ***-2 cell lines confrm the cellular uptake and trans-epithelial *** conclusion,a fraction of SMEDDSs can survive the lipolysis in the gastrointestinal tract,permeate across the epithelia,translocate via the lymph,and accumulate mainly in the liver.

关键词： SMEDDS In vivo fate lipolysis Trans-epithelial transport Lymph Aggregation-caused quenching Caco-2 Absorption

Changes of protein oxidation,lipid oxidation and lipolysis in Chinese dry sausage with different sodium chloride curing salt content

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Food Science and Human Wellness 2020年第4期9卷 328-337页

作者： Bing Zhao Huimin Zhou Shunliang Zhang Xiaoqian Pan Su Li Ning Zhu Qianrong Wu Shouwei Wang Xiaoling Qiao Wenhua Chen China Meat Research Centre Beijing100068China Beijing Academy of Food Sciences Beijing100068China

The effect of sodium chloride(NaCl)curing salt content on protein oxidation,lipid oxidation and lipolysis of Chinese dry sausage was *** groups Chinese dry sausages with 2%and 4%(m/m)salt content were *** degree of protein oxidation increased during the processes in two groups sausages,while the content of phospholipids decreased,neutral lipids and free fatty acids *** degree of protein oxidation,lipid oxidation and lipolysis in 4%NaCl content group was higher than those in 2%NaCl content group,while 4%NaCl content group has higher lipase *** conclusion,4%NaCl may facilitate the protein oxidation,lipid hydrolysis and oxidation in Chinese dry sausage,and the protein oxidation had strong correlation with lipid oxidation and *** results could provide a basis for improving the technology of industrial production.

关键词： Chinese dry sausage sodium chloride Protein oxidation Lipids oxidation lipolysis

Gastrointestinal digestive fate of whey protein isolate coated liposomes loading astaxanthin:lipolysis,release,and bioaccessibility

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Food Bioscience 2022年第1期45卷 115-121页

作者： Li Pan Hua Li Lifen Hou Zhengang Chang Yuanyuan Li Xueqin Li College of Food Science and Engineering Henan University of TechnologyZhengzhouChina Department of International Trade Henan Jingu Industry Development Co.LtdZhengzhouChina School of Chemistry and Chemical Engineering Henan University of TechnologyZhengzhouChina

Whey protein isolate coated astaxanthin-loaded liposomes were prepared in this *** gastrointestinal digestive fate of whey protein isolate coated astaxanthin-loaded liposomes was evaluated in terms of particle size,size distribution,zeta potential,and morphology during in vitro digestion as a function of *** on the particle size and morphology of whey protein isolate coated astaxanthin-loaded liposomes showed that the majority of particles maintained spherical shape with a progressive increase of particle size after passage through the *** zeta potential on whey protein isolate coated astaxanthin-loaded liposomes became highly negative after *** compared in uncoated liposomes,the astaxanthin release in whey protein isolate coated liposomes was slower in simulated gastric fluid digestion,while was faster in simulated intestinal fluid *** in vitro digestion,the bioaccessibility of astaxanthin was improved significantly after whey protein isolate *** was also found that the whey protein isolate coating could protect liposomes against destruction and suppress the lateral mobility of pyrene,resulting in lower micropolarity and fluidity of liposomal membrane during the *** findings may guide the potential application of whey protein isolate coated liposomes for improving the bioaccessibility and stability of astaxanthin in nutraceuticals and pharmaceutics.

关键词： Astaxanthin Liposomes Whey protein isolate coating Release lipolysis Bioaccessibility

β-Lactoglobulin stabilized lipid nanoparticles enhance oral absorption of insulin by slowing down lipolysis

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Chinese Chemical Letters 2024年第4期35卷 287-291页

作者： Lu Li Suticha Chunta Xianzi Zheng Haisheng He Wei Wu Yi Lu School of Pharmacy&Key Laboratory of Smart Drug Delivery of MOE Fudan UniversityShanghai 201203China Department of Clinical Chemistry Faculty of Medical TechnologyPrince of Songkla UniversitySongkhla 90110Thailand Shanghai Skin Disease Hospital Tongji University School of MedicineShanghai 200433China Fudan Zhangjiang Institute Shanghai 201203China

Lipid-based nanocarriers have staged a remarkable comeback in the oral delivery of proteins and peptides, but delivery efficiency is compromised by lipolysis. β-Lactoglobulin(β-lg) stabilized lipid nanoparticles, including nanoemulsions(NE@β-lg) and nanocapsules(NC@β-lg), were developed to enhance the oral absorption of insulin by slowing down lipolysis due to the protection from β-lg. Cremophor EL stabilized nanoemulsions(NE@Cre-EL) were prepared and set as a control. The lipid nanoparticles produced mild and sustained hypoglycemic effects, amounting to oral bioavailability of 3.0% ± 0.3%, 7.0% ± 1.1%, and7.7% ± 0.8% for NE@Cre-EL, NE@β-lg, and NC@β-lg, respectively. Aggregation-caused quenching(ACQ)probes enabled the identification of intact nanoparticles, which were used to investigate the in vivo and intracellular fates of the lipid nanoparticles. In vitro digestion/lipolysis and ex vivo imaging confirmed delayed lipolysis from β-lg stabilized lipid nanoparticles. NC@β-lg was more resistant to intestinal lipolysis than NE@β-lg due to the Ca^(2+)-induced crosslinking. Live imaging revealed the transepithelial transport of intact nanoparticles and their accumulation in the liver. Cellular studies confirmed the uptake of intact nanoparticles. Slowing down lipolysis via food proteins represents a good strategy to enhance the oral absorption of lipid nanoparticles and thus co-formulated biomacromolecules.

关键词： β-Lactoglobulin Lipid nanoparticles Oral absorption Insulin lipolysis Aggregation-caused quenching

Lacticaseibacillus paracasei K56 inhibits lipid accumulation in adipocytes by promoting lipolysis

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Food Science and Human Wellness 2024年第6期13卷 3511-3521页

作者： Silu Wang Jinxing Li Wei-Hsien Liu Niya Li Huijing Liang Weilian Hung Qiuyue Jiang Ruyue Cheng Xi Shen Fang He Department of Nutrition and Food Hygiene West China School of Public Health and West China Fourth HospitalSichuan UniversityChengdu 610041China Inner Mongolia Yili Industrial Group Co. Ltd.Hohhot 010110China

Probiotics crosstalk immunity to improve host glycolipid metabolism,which is a new strategy for *** study aimed to explore the functions of Lacticaseibacillus paracasei K56(K56),in regulating the lipid metabolism of adipocytes and its immune regulation *** co-cultured RAW264.7 macrophages with K56,and the K56-stimulated RAW264.7-conditioned medium(K56-CM)was collected and treated with 3T3-L1 *** expression of lipid metabolism-related markers of adipocytes,and the content of cytokines in CMs were *** results demonstrated that K56-CM promoted the expression of peroxisome proliferator-activated receptor-γ(PPAR-γ),CCAAT/enhancer binding proteinα(C/EBPα),and other adipogenesis-related markers,which resulted in the upregulation of lipolysis markers,such as hormone-sensitive lipase(HSL),adipose triglyceride lipase(ATGL).The activation of lipolysis enhanced the expression of fatty acidβ-oxidation-related and mitochondrial biogenesis-related markers and reduced lipid accumulation in *** glycolipid metabolism pattern of K56 may be due to its immunomodulatory characteristics,which stimulated macrophages to secrete fewer TNF-α,thereby promoting the expression of lipolysis-related markers,and TNF-αsynergized with lipase to promote *** has not been reported that *** modulated lipid metabolism via the lipolysis pathway,which suggested that K56 may regulate glycolipid metabolism of the host by maintaining immune homeostasis.

关键词： Obesity Lacticaseibacillus Macrophage Adipocyte lipolysis

Korean Chungtaejeon tea extract attenuates weight gain in C57BL/6J-Lep ob/ob mice and regulates adipogenesis and lipolysis in 3T3-L1 adipocytes

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Journal of Integrative Medicine 2017年第1期15卷 56-63页

作者： Bhesh Raj Sharma Dong Wook Kim Dong Young Rhyu Department of Oriental Medicine Resources and Institute of Korean Medicine Industry Mokpo NationalUniversity Jeonnam 534-729 Republic of Korea

OBJECTIVE： Traditional Korean Chungtaejeon （CTJ） tea is a type of fermented tea, which has received increasing attention in recent years because of its purported health benefits. The present study was designed to investigate the effect and mechanism of CTJ tea extract on body weight gain using C57BL/6J-Lep ob/ob mice and 3T3-L1 adipocytes, respectively. METHODS： The effects of CTJ on cell viability, lipid accumulation, and expression of protein and mRNA were measured in 3T3-L1 adipocytes by using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, oil red O staining, Western blotting, and reverse transcriptase-polymerase chain reaction analyses. C57BL6J-Lep ob/ob mice were administered with CTJ （200 or 400 mg/kg body weight） for ten weeks. Then, body weight, food intake, total cholesterol, and triglyceride were measured in ob/ob mice. RESULTS： CTJ tea extract treated at 250 wg/mL （CTJ250） significantly suppressed lipid accumulation in the differentiated 3T3-L1 adipocytes. Likewise, CTJ250 significantly decreased the protein expression of peroxisome proliferator-activated receptor Y （PPARy）, CCJ^u~,T/enhancer-binding protein a, and adipocyte lipid- binding protein, and regulated the mRNA expression of PPART, sterol regulatory element-binding protein- lc gene, fatty acid synthase, adipocyte lipid-binding protein, hormone-sensitive lipase, carnitine palmitoyl transferase 1, cluster of differentiation 36, and acetyl-CoA carboxylase in the differentiated 3T3-L1 adipocytes. Mice administered with CTJ showed dose-dependent decrease in body weight gain, starting from week 4 of the experiment. CTJ tea extract administered at 400 mg/kg body weight significantly decreased fat mass, food efficacy ratio, and levels of plasma triglyceride and total cholesterol. CONCLUSION： CTJ attenuated weight gain in ob/ob mice and regulated the activity of the molecules involved in adipogenesis and lipolysis in 3T3-L1 adipocytes. CTJ is a potentially valuable herbal therapy for the p

关键词： adipogenesis Chungtaejeon lipolysis C57BL/6J-Lep ob/ob mice weight loss